A6691

Amidase aus Pseudomonas aeruginosa

recombinant, expressed in E. coli, buffered aqueous glycerol solution, hydroxamate transferase ≥200 units/mg protein (biuret)

• Synonym(e): Acrylamid-Amidohydrolase, Acylase
• CAS-Nummer: 9012-56-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.5.1.4 (BRENDA, IUBMB)
• MDL number: MFCD00166319
• Recombinant: expressed in E. coli
• Concentration: 14 mg/mL

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: buffered aqueous glycerol solution
• hydroxamate transferase activity: ≥200 units/mg protein (biuret)
• concentration: 14 mg/mL
• UniProt accession no.: Q9HWL8
• storage temp.: −20°C
• Gene Information: Pseudomonas aeruginosa PAO1 ... PA4163(880181)

Beschreibung

Application

The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .

Amidase from Pseudomonas aeruginosa has been used for testing its capability to hydrolyze ochratoxin A.

Biochem/physiol Actions

The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .

Physical form

Lösung in 50% Glycerin, enthält Spuren von 2-Mercaptoethanol und Phosphat-Puffersalz

Other Notes

1 U setzt 1.0 μ;mol Acetamid und Hydroxylamin zu Acetohydroxamat und Ammoniak pro Minute um bei pH 7.2 und 37°C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)