Antifoam SE-15 is a 10% emulsion of active silicone polymer and non-ionic emulsifiers. It can be repeatedly sterilized by autoclaving. Antifoam C Emulsion is an aqueous emulsion containing 30% active silicone. Further details about the different properties can be found in the datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/313/a8582pis.pdf
A8582
Antifoam SE-15
aqueous emulsion for bacterial and mammalian systems
Synonym(e):
silicone-based antifoam
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100 G
€ 54,80
500 G
€ 200,00
Über diesen Artikel
UNSPSC-Code:
12161901
NACRES:
NA.85
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Unterstützung erhaltenQualitätsniveau
Form
emulsion (aqueous)
Konzentration
10% (active silicon)
Methode(n)
cell culture | hybridoma: suitable
Löslichkeit
H2O: soluble (effective hot or cold.)
Dichte
1.0 g/mL at 25 °C
Anwendung(en)
microbiology
Lagertemp.
room temp
Eignung
(Mammalian (suspension))
bacteria (fermentation)
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Dieser Artikel | A6582 | A8311 | A5757 |
|---|---|---|---|
| suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability (Mammalian (suspension)), bacteria (fermentation) | suitability - |
| application(s) microbiology | application(s) microbiology | application(s) microbiology | application(s) - |
| form emulsion (aqueous) | form emulsion (aqueous) | form emulsion (aqueous) | form emulsion |
| technique(s) cell culture | hybridoma: suitable | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable | technique(s) - | technique(s) cell culture | hybridoma: suitable, microbiological culture: suitable |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. - |
| solubility H2O: soluble (effective hot or cold.) | solubility - | solubility - | solubility - |
Allgemeine Beschreibung
Antifoam SE-15 is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production.
Anwendung
Antifoam SE-15 has been used:
- in the preparation of ramified rolling circle amplification (RAM) amplification buffer.[1]
- to find its effects on cell viability, virus infectivity and PCR (polymerase chain reaction) performance.[2]
- as a constituent of phosphate-buffered saline (PBS) for the determination of total antioxidant status (TAOS) by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay. [3]
- as a component of autoinduction expression culture. [4]
- as a component of cyclic adenosine monophosphate (cAMP) detection buffer. [5]
Leistungsmerkmale und Vorteile
- Controls foaming in microbial culture flasks or fermentations
- Non-toxic to the culture medium
- Can be pumped to fermenter on an as-needed basis
Angaben zur Herstellung
Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted
suspensions should be used immediately.
suspensions should be used immediately.
Sonstige Hinweise
Antifoam SE-15 is an aqueous emulsion containing 10% active silicon and non-ionic emulsifiers .
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James H Smith et al.
PloS one, 8(5), e65053-e65053 (2013-06-01)
Isothermal amplification methods provide alternatives to PCR that may be preferable for some nucleic acid target detection tasks. Among current isothermal target detection methods, ramified rolling circle amplification (RAM) of single-stranded DNA circles that are formed by ligation of linear
Detection of nucleic acid targets using ramified rolling circle DNA amplification: a single nucleotide polymorphism assay model
Smith J H and Beals T P
PLoS ONE, 8(5), e65053-e65053 (2013)
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Verwandter Inhalt
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細胞培養液に添加するためにアンチフォームSE-15の購入を検討しております。この商品は、オートクレーブ可能でしょうか、オートクレーブ可能の場合何回程度が限度でしょうか。 もし可能であれば、アンチフォーム C エマルジョンとの違いも教えていただけないでしょうか。 （アンチフォーム C エマルジョンもオートクレーブ可能でしょうか。） よろしくお願い申し上げます。
1 answer-
Helpful?
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At what concentration is SE-15 (A8582) to be used in bacterial cell culture to control foam?
1 answer-
This Antifoam product is typically used at starting concentrations of 0.01 - 0.02%. Optimization may be required based on variable culture density, ingredients, and conditions. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/133/175/a8582dat.pdfHelpful?
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I was wondering if you had any information in regards to the stability of antifoam SE-15, A8582. In particular, do you have any data about the stability of this product at pH 8.0? When in a solution, do you have any data on the product stability?
1 answer-
This product is stable at room temperature. Antifoam SE-15 can be pre-diluted with 3-10 parts of cool water to aid in dispersion. Pre-diluted suspensions should be used immediately. The stability of this product is not tested at pH 8.0. If you are looking for antifoams that will be stable at pH 8.0, perhaps you may consider Antifoam A Concentrate (Product A5633 and A6582), They are stable at pH 5.0-9.0. Please see this link for any additional information on Product A8582: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/316/313/a8582pis.pdf
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