N3786

α(2→3,6,8,9) Neuraminidase aus Arthrobacter ureafaciens

Proteomics Grade, suitable for MALDI-TOF MS

• Synonym(e): Neuraminidase aus Arthrobacter ureafaciens, Acyl-Neuraminyl-Hydrolase, Sialidase
• CAS-Nummer: 9001-67-6
• UNSPSC Code: 12352204
• NACRES: NA.32
• EC Number: 232-624-6
• MDL number: MFCD00131711
• EG-Nummer: 3.2.1.18 (BRENDA, IUBMB)
• Specific activity: ≥25 U/vial

Eigenschaften

• form: lyophilized powder
• Quality Segment: 200
• quality: Proteomics Grade
• specific activity: ≥25 U/vial
• suitability: suitable for MALDI-TOF MS
• storage temp.: 2-8°C

Beschreibung

Application

Neuraminidase is an important deglycosylation enzyme capable of cleaving all non-reducing unbranched N-acetylneuraminic and N-glycolylneuraminic acid residues by hydrolysis of α(2→6), α(2→3), α(2→8), and α(2→9) linkages (affinity in the order given). Branched sialic acids may also be cleaved with the use of high concentrations of enzyme and prolonged incubations. Desialylated glycoproteins may then be further characterized by treatment with various exoglycosidases resulting in partial or complete O-deglycosylation. SDS-PAGE and MALDI-TOF MS are typically utilized in purification, structural analysis, and sequencing process. These techniques also remove heterogeneity and charge from the glycoprotein.

Packaging

Provided with 5× concentrate reaction buffer.

Physical form

Lyophilized powder

Analysis Note

The buffer solution has been specifically formulated to assure maximum enzyme stability and assay sensitivity.

Other Notes

One unit will release 1 nmole of 4-methylumbelliferone from 2-(4-methylumbelliferyl) α-D-N-acetylneuraminic acid per minute at pH 5.5 at 37° C.

Kit-Komponenten auch einzeln erhältlich

• N3536

  Neuraminidase 5X Reaction Buffer 1.5 mL

  SDB

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable