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Technisches Datenblatt

Produktname
MystiCq® microRNA-cDNA-Synthese-Mischung

Produktnummer
mirrt

Produktmarke
sigma

CAS-Nummer

Molekulargewicht

Test

Spezifikation

qScript microRNA cDNA Synthesis KitMust have detection of microRNA from 100 ng to 10 pg.Correlation of determination (R2) ≥ 0.990 from Ct standard curve analysis.Slope from Ct standard curve analysis between -3.20 and -3.75.The +PAP reactions must show a ≥ 11 Ct difference from the “no-PAP” reactions.There must be at least three negative template controls with “undetermined” values.
DNaseDNase activity must be below the detectable limits of 100 pg DNase I equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration.
RNaseRNase activity must be below the detectable limits of 1 pg RNase A equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration
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