- Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.
Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes.
STAR protocols (2020-10-29)
Mandy Kleinsorge, Lukas Cyganek
PMID33111079
ZUSAMMENFASSUNG
The generation of homogeneous populations of subtype-specific cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs) is crucial in cardiovascular disease modeling as well as in drug discovery and cardiotoxicity screenings. This protocol describes a simple, robust, and efficient monolayer-based differentiation of hiPSCs into defined atrial and ventricular cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Cyganek et al., 2018.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Monoklonales Anti-α-Aktinin (Sarkomer) in Maus hergestellte Antikörper, clone EA-53, ascites fluid
Sigma-Aldrich
Y-27632-CAS 331752-47-7-Calbiochem, Y-27632A, CAS 331752-47-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.
Sigma-Aldrich
Wnt-Antagonist II, IWP-2 – CAS 686770-61-6 – Calbiochem, The Wnt Antagonist II, IWP-2, also referenced under CAS 686770-61-6, controls the biological activity of Wnt. This small molecule/inhibitor is primarily used for Cancer applications.
Sigma-Aldrich
Anti-RYR2 in Kaninchen hergestellte Antikörper, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution