- Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines.
Evaluation of Tegaran Formula ZhenHua cytotoxicity against human cancer cell lines.
PloS one (2020-10-22)
Panagiotis Parsonidis, Ioanna Vlachou, Alexandra Mamagkaki, Ioannis Bouris, Vasiliki Daikopoulou, Ioannis Papasotiriou
PMID33085705
ZUSAMMENFASSUNG
The aim of this study is to evaluate the potential health effects of Tegaran Formula ZhenHua, a nutritional supplement used mainly by cancer patients. Its active ingredients and cytotoxicity was assessed with analytical methods and viability assays, respectively. The analytical methods consisted of dissolution, disintegration, HPLC, LC/MS, GC/MS and NMR. Cytotoxicity was assessed by MTT, SRB, CVE colorimetric viability assays in 0, 24, 48 and 72h time points. The results indicate that Tegaran Formula ZhenHua supplement did not present any cytotoxic effects due to issues related to the capsules' solubility, distribution and identification of the active ingredient.
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Phosphatgepufferte Kochsalzlösung -Lösung, powder, pH 7.4, for preparing 1 L solutions
Sigma-Aldrich
L-Glutamin -Lösung, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
RPMI-1640-Medium, With sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
MEM Nicht-essentielle Aminosäure-Lösung (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium, niedriger Glucosegehalt, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture