Biodegradation of tetrahydrofuran and 1,4-dioxane by soluble diiron monooxygenase in Pseudonocardia sp. strain ENV478.

Biodegradation of tetrahydrofuran and 1,4-dioxane by soluble diiron monooxygenase in Pseudonocardia sp. strain ENV478.

Journal of molecular microbiology and biotechnology (2012-11-14)

Hisako Masuda, Kevin McClay, Robert J Steffan, Gerben J Zylstra

ZUSAMMENFASSUNG

1,4-Dioxane is an important groundwater contaminant. Pseudonocardia sp. strain ENV478 degrades 1,4-dioxane via cometabolism after the growth on tetrahydrofuran (THF) and other carbon sources. Here, we have identified a THF monooxygenase (thm) in ENV478. The thm genes are transcribed constitutively and are induced to higher levels by THF. Decreased translation of the thmB gene encoding one of the monooxygenase subunits by antisense RNA resulted in the loss of its ability to degrade THF and 1,4-dioxane. This is the first study to link thm genes to THF degradation, as well as the cometabolic oxidation of 1,4-dioxane.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

34865

Sigma-Aldrich

Tetrahydrofuran, inhibitor-free, suitable for HPLC, ≥99.9%

360589

Sigma-Aldrich

Tetrahydrofuran, contains 200-400 ppm BHT as inhibitor, ACS reagent, ≥99.0%

401757

Sigma-Aldrich

Tetrahydrofuran, anhydrous, ≥99.9%, inhibitor-free

186562

Sigma-Aldrich

Tetrahydrofuran, anhydrous, contains 250 ppm BHT as inhibitor, ≥99.9%

360481

Sigma-Aldrich

1,4-Dioxan, ACS reagent, ≥99.0%, contains ≤25 ppm BHT as stabilizer

34857

Sigma-Aldrich

1,4-Dioxan, suitable for HPLC, ≥99.5%

676934

Sigma-Aldrich

1,4-Dioxan, ACS reagent, ≥99.0%, contains <=25 ppm BHT as stabilizer

676764

Sigma-Aldrich

Tetrahydrofuran, ACS reagent, ≥99.0%, contains 200-400 ppm BHT as inhibitor

76887

Supelco

1,4-Dioxan, analytical standard

CRM48367

Supelco

1,4-Dioxan-Lösung, certified reference material, 2000 μg/mL in methanol, ampule of 1 mL

270385

Supelco

Tetrahydrofuran, HPLC grade, ≥99.9%, inhibitor-free

78445

Supelco

Tetrahydrofuran, analytical standard

551368

Sigma-Aldrich

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D)

87369

Supelco

Tetrahydrofuran, Selectophore^™, ≥99.5%

736171

Supelco

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), NMR tube size 5 mm × 8 in.

704814

Sigma-Aldrich

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 10 mM in D₂O ("100%", 99.96 atom % D), NMR tube size 5 mm × 7 in.

684228

Sigma-Aldrich

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), NMR tube size 10 mm × 8 in.

612073

Sigma-Aldrich

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), chromium(III) acetylacetonate 5 mg/mL, NMR tube size 3 mm × 8 in.

611905

Sigma-Aldrich

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 40% in benzene-d₆ (99.6 atom % D), chromium(III) acetylacetonate 5 mg/mL, NMR tube size 5 mm × 8 in.

631868

Supelco

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 5 mM in chloroform-d (99.8 atom % D), NMR tube size 3 mm × 8 in.

631833

Supelco

1,4-Dioxan -Lösung, suitable for NMR (reference standard), 5 mM in benzene-d₆ (99.6 atom % D), NMR tube size 3 mm × 8 in.