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  • [Determination of the relative RNA-content of liver cell nuclei with gallocyanin-chromealum-staining (author's transl)].

[Determination of the relative RNA-content of liver cell nuclei with gallocyanin-chromealum-staining (author's transl)].

Acta histochemica (1981-01-01)
K Wenzelides, G Korek, K Voss
ZUSAMMENFASSUNG

The relative RNA-content of liver cell nuclei from histological slides of rat liver was determined by automatical microscope picture analysis. The slides were stained with Gallocyanin-Chromealum. Extinction measurements are made before and after perchloracid extraction of RNA for the same slide. The changes of extinction measurements caused by the changes of slide thickness and of tissue inhomogeneity could be lowered using this method.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Chrom(III)-kaliumsulfat Dodecahydrat, ACS reagent, ≥98%
Sigma-Aldrich
Gallocyanin, Dye content 90 %