P3296

Protein G Sepharose^™, Fast Flow

Name: Protein G Sepharose<SUP>™</SUP>, Fast Flow
Brand: SIGMA
Price: 568 AUD

recombinant, expressed in E. coli, aqueous ethanol suspension

Protein G Sepharose™, Fast Flow

Synonym(s):

Protein G-Agarose, Fast Flow from Streptococcus sp.

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1 ML

$568.00

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About This Item

MDL number:

MFCD00165580

UNSPSC Code:

41106500

NACRES:

NA.56

Key Documents

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recombinant

expressed in E. coli

form

aqueous ethanol suspension

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

extent of labeling

~2 mg per mL

technique(s)

affinity chromatography: suitable

matrix

Sepharose 4B Fast Flow

matrix activation

cyanogen bromide

matrix attachment

amino

matrix spacer

1 atom

storage temp.

2-8°C

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Related Categories

Antibody Protein Purification

Protein Purification Chromatography

Protein Sample Prep

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Show Differences

1 of 4

This Item	P9424	P3391	P8170
Millipore P3296 Protein G Sepharose^™, Fast Flow	Millipore P9424 ProteinA-Sepharose^™ 4, Fast Flow from Staphylococcus aureus	Millipore P3391 Protein A-Sepharose^™ from Staphylococcus aureus	Sigma-Aldrich P8170 Protein G−Peroxidase from Streptococcus sp.
technique(s) affinity chromatography: suitable	technique(s) affinity chromatography: suitable	technique(s) affinity chromatography: suitable	technique(s) direct ELISA: 1:100,000 using human IgG
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant expressed in unspecified host
form aqueous ethanol suspension	form aqueous ethanol suspension	form lyophilized powder	form lyophilized powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. −20°C
matrix Sepharose 4B Fast Flow	matrix Sepharose 4B Fast Flow	matrix Sepharose CL-4B	matrix -
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200

General description

Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148.[1] This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200.[2] The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10.[3] It acts as a powerful reagent for the detection of IgG.[1]

P3296-5Ml′s updated product number is GE17-0618-01

Application

Protein G-Sepharose^™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose^™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.

Physical form

Suspension in 20% ethanol

Preparation Note

Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted

Legal Information

Sepharose is a trademark of Cytiva

related product

Product No.

Description

Pricing

P4691

Protein G-Agarose, Fast Flow from Streptococcus sp., saline suspension

GE17-0618-01

Protein G Sepharose^™ 4 Fast Flow, Cytiva 17-0618-01, pack of 5 mL

Pictograms

GHS02

Signal Word

Warning

Hazard Statements

H226

Precautionary Statements

P210 - P233 - P240 - P241 - P242 - P243

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

115.0 °F - closed cup

Flash Point(C)

46.1 °C - closed cup

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Histone deacetylase inhibitors induce the degradation of the t(8;21) fusion oncoprotein.

G Yang et al.

Oncogene, 26(1), 91-101 (2006-06-27)

The t(8;21) chromosomal translocation that generates the fusion oncoprotein RUNX1-ETO predominates in leukemia patients of the French-American-British (FAB) class M2 subtype. The oncoprotein has the capacity to promote expansion of hematopoietic stem/progenitor cells and induces leukemia in association with other

Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.

Cristina Hidalgo-Carcedo et al.

Nature cell biology, 13(1), 49-58 (2010-12-21)

Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin

A physicochemical study of protein G, a molecule with unique immunoglobulin G-binding properties.

B Akerström et al.

The Journal of biological chemistry, 261(22), 10240-10247 (1986-08-05)

Protein G, an IgG-binding molecule, was prepared from the cell walls of a group G streptococcal strain, G-148. The protein could be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex

Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies.

B Akerström et al.

Journal of immunology (Baltimore, Md. : 1950), 135(4), 2589-2592 (1985-10-01)

Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein recently isolated from group G streptococci. We have investigated the avidity of protein G for various monoclonal and polyclonal Ig of the IgG class, and compared it with the binding

The interaction between SPARC and GRP78 interferes with ER stress signaling and potentiates apoptosis via PERK/eIF2α and IRE1α/XBP-1 in colorectal cancer.

Yi-Jye Chern et al.

Cell death & disease, 10(7), 504-504 (2019-06-28)

Therapy-refractory disease is one of the main contributors of treatment failure in cancer. In colorectal cancer (CRC), SPARC can function as a sensitizer to conventional chemotherapy by enhancing apoptosis by interfering with the activity of Bcl-2. Here, we examine a

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Immunoprecipitation Procedure

Techniques for protein antigen molecular weight determination, protein interactions, enzymatic activity, and post-translational modifications.

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