1.52028
Chromolith® NH2 (monolithic) HPLC Columns
L × I.D. 100 mm x 4.6 mm HPLC Column
Synonym(s):
Chromolith® Performance NH2 100-4.6 HPLC column
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€999.00
€999.00
Estimated to ship on02 February 2026Details
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Product Name
Chromolith® NH2, L × I.D. 100 mm x 4.6 mm HPLC column
material
PEEK column
Quality Level
Agency
suitable for USP L8
product line
Chromolith®
feature
endcapped: no
packaging
1 ea of
extent of labeling
5% Carbon loading
parameter
200 bar pressure
45 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
10 cm × 4.6 mm
matrix
monolithic silica
matrix active group
NH2 bonding phase
pore size
13 nm mesoporosity
2 μm macroporosity
operating pH range
2-7.5
separation technique
hydrophilic interaction (HILIC)
normal phase
reversed phase
storage temp.
no temp limit
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Related Categories
1 of 4
This Item | 1.52026 | 1.52027 | 1.52048 |
|---|---|---|---|
| separation technique hydrophilic interaction (HILIC), reversed phase, normal phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase | separation technique hydrophilic interaction (HILIC), normal phase, reversed phase |
| matrix active group NH2 bonding phase | matrix active group NH2 bonding phase | matrix active group NH2 bonding phase | matrix active group Cyano (CN) bonding phase |
| technique(s) HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium), LC/MS: suitable | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium) | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (Low - Medium) | technique(s) HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High) |
| product line Chromolith® | product line Chromolith® | product line Chromolith® | product line Chromolith® |
| matrix monolithic silica | matrix monolithic silica | matrix monolithic silica | matrix monolithic silica |
| pore size 13 nm mesoporosity, 2 μm macroporosity | pore size 13 nm mesoporosity, 2 μm macroporosity | pore size 13 nm mesoporosity, 2 μm macroporosity | pore size 13 nm mesoporosity, 2 μm macroporosity |
General description
Chromolith® NH2 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å). Chromolith® NH2 columns fall under the USP L8 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® NH2 columns are suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for separations of complex samples on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® NH2 5-4.6 guard cartridges (3 pieces) [1.52030 with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52030]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Analysis Note
Performance (2-nitroanisol)
Tailing (USP): 0.85 - 1.30 %
Theoretical Plates (N/m) (Cytosine): ≥ 80000
Capacity factor (Cytosine): ≥ 1.3
Legal Information
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Articles
A new HPLC-RI method is proposed for the analysis of Lactose in milk using a Chromolith® NH2 column.
Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.
This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.
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