324796

Lys-C Protease

Cleaves at lysine residues, suitable for mass spectrometry, from Achromobacter lyticus

• Synonym(s): Endopeptidase Lys-C, Achromobacter lyticus
• CAS Number: 78642-25-8
• MDL number: MFCD00130333
• UNSPSC Code: 12352202

Properties

• Product Name: Endopeptidase Lys-C, Achromobacter lyticus,
• form: lyophilized
• Quality Level: 100
• specific activity: ≥2 AU/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• technique(s): mass spectrometry (MS): suitable
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Native endopeptidase Lys-C from Achromobacter lyticus. Lysyl endopeptidase is a serine protease secreted by soil bacteria and specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues. Optimal pH: 9.0-9.5.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.

Reconstitute 1 vial with 1 ml solvent; if the enzyme is not completely solubilized, add 1-3 ml additional solvent until completely dissolved.

Other Notes

Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

One AU (amidase unit) is defined as the amount of enzyme that catalyzes the release of 1 µM pNA from N-benzoyl-DL-lysine-pNA per min at 30°C, pH 9.5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)