AP1064

PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb

Name: PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb
Brand: MM
Price: 387 EUR
Availability: InStock

liquid, Calbiochem^®

Synonym(s):

Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb

Slide 1 of 1

Select a Size

50 μG

€387.00

Estimated to ship TODAYDetails

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

Key Documents

View All Documentation

biological source

rabbit

Quality Level

200

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer300)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item	AP1063	DR1025	506119
Sigma-Aldrich AP1064 PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb	Sigma-Aldrich AP1063 PhosphoDetect Anti-PDH-E1α (pSer²³²) Rabbit pAb	Sigma-Aldrich DR1025 PhosphoDetect Anti-Chk1 (pSer³¹⁷) Rabbit pAb	Sigma-Aldrich 506119 PhosphoDetect Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb
antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody	antibody form affinity isolated antibody
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 100
biological source rabbit	biological source rabbit	biological source rabbit	biological source rabbit
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
form liquid	form liquid	form liquid	form liquid
shipped in wet ice	shipped in wet ice	shipped in wet ice	shipped in wet ice

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰.

Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser³⁰⁰ in HEK293 cells.

This PhosphoDetect Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α

Application

Immunoblotting (0.5 μg/ml)
Immunocytochemistry (1 μg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
HEK293 cells treated with dichloroacetate

Positive Control
HEK293 cells

Other Notes

Due to a high degree of sequence identity, this antibody is also expected to cross-react with mouse, rat, bovine, Zebra fish, and Xenopus phospho-PDH-E1α Antibody should be titrated for optimal results in individual systems.

Rardin M.J., et. al. 2009. Anal. Biochem.2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans.34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Not finding the right product?

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Unusual Bicyclo[3.2.1]Octanoid Neolignans from Leaves of Piper crocatum and Their Effect on Pyruvate Dehydrogenase Activity.

Yu Juan Chai et al.

Plants (Basel, Switzerland), 10(9) (2021-09-29)

Three undescribed bicyclo[3.2.1]octanoid neolignan glucosides, along with a known neolignan, were isolated from the leaves of Piper crocatum Ruiz & Pav. Their chemical structures were elucidated using extensive spectroscopic analyses including 1D and 2D NMR experiments and HR-ESI-MS analysis, as

Skeletal Muscle Pyruvate Dehydrogenase Phosphorylation and Lactate Accumulation During Sprint Exercise in Normoxia and Severe Acute Hypoxia: Effects of Antioxidants.

David Morales-Alamo et al.

Frontiers in physiology, 9, 188-188 (2018-04-05)

Compared to normoxia, during sprint exercise in severe acute hypoxia the glycolytic rate is increased leading to greater lactate accumulation, acidification, and oxidative stress. To determine the role played by pyruvate dehydrogenase (PDH) activation and reactive nitrogen and oxygen species

Switching off IMMP2L signaling drives senescence via simultaneous metabolic alteration and blockage of cell death.

Lifeng Yuan et al.

Cell research, 28(6), 625-643 (2018-05-29)

Cellular senescence is a fundamental cell fate playing a significant role throughout the natural aging process. However, the molecular determinants distinguishing senescence from other cell-cycle arrest states such as quiescence and post-mitotic state, and the specified mechanisms underlying cell-fate decisions

BCAA catabolism in brown fat controls energy homeostasis through SLC25A44.

Takeshi Yoneshiro et al.

Nature, 572(7771), 614-619 (2019-08-23)

Branched-chain amino acid (BCAA; valine, leucine and isoleucine) supplementation is often beneficial to energy expenditure; however, increased circulating levels of BCAA are linked to obesity and diabetes. The mechanisms of this paradox remain unclear. Here we report that, on cold exposure

Secondary Metabolites of The Endophytic Fungus Alternaria alternata JS0515 Isolated from Vitex rotundifolia and Their Effects on Pyruvate Dehydrogenase Activity.

Changyeol Lee et al.

Molecules (Basel, Switzerland), 24(24) (2019-12-11)

The fungal strain Alternaria alternata JS0515 was isolated from Vitex rotundifolia (beach vitex). Twelve secondary metabolites, including one new altenusin derivative (1), were isolated. The isolated metabolites included seven known altenusin derivatives (2-8), two isochromanones (9, 10), one perylenequinone (11)

View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service