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Merck

01696

Acrylamide

for Northern and Southern blotting, powder blend

Synonym(s):

2-Propenamide, Polyacrylamide, acrylic amide, akrylamid, Acrylic acid amide

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€116.00

500 G

€343.00

1 KG

€592.00

€116.00

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About This Item

Linear Formula:
CH2=CHCONH2
CAS Number:
79-06-1
Molecular Weight:
71.08
NACRES:
NA.25
PubChem Substance ID:
329747346
eCl@ss:
39031205
UNSPSC Code:
41105319
EC Number:
201-173-7
MDL number:
MFCD00008032
Beilstein/REAXYS Number:
605349

Key Documents

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Product Name

Acrylamide, for Northern and Southern blotting, powder blend

InChI key

HRPVXLWXLXDGHG-UHFFFAOYSA-N

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

SMILES string

NC(=O)C=C

grade

Molecular Biology

vapor density

2.45 (vs air)

vapor pressure

0.03 mmHg ( 40 °C)

product line

BioReagent

assay

≥99.5% (GC)

form

powder blend

technique(s)

electrophoresis: suitable

impurities

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

solubility

H2O: 0.25 g/mL at 20 °C, clear, colorless

storage temp.

2-8°C

Quality Level

200

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This Item
017001.107848.00830
Acrylamide for Northern and Southern blotting, powder blend

Sigma-Aldrich

01696

Acrylamide

Acrylamide purum, ≥98.0% (GC)

Sigma-Aldrich

01700

Acrylamide

Acrylamide for electrophoresis

Millipore

1.10784

Acrylamide

Acrylamide for synthesis

Sigma-Aldrich

8.00830

Acrylamide

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

powder blend

form

-

form

solid

form

solid

solubility

H2O: 0.25 g/mL at 20 °C, clear, colorless

solubility

water: soluble 200 g/L at 20 °C

solubility

2040 g/L

solubility

2040 g/L

grade

for molecular biology

grade

-

grade

-

grade

-

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-30°C

storage temp.

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Suitable for making polyacrylamide gels according to established recipes.

Disclaimer

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

related product

Product No.
Description
Pricing

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

280.4 °F - closed cup

flash_point_c

138 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN2037
BCCK3074
BCCH8174
BCCH0715
BCCJ5814

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R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise
Jonas S Laursen et al.
Journal of the American Chemical Society, 135(7), 2835-2844 (2013-01-25)
Non-natural peptide analogs have significant potential for the development of new materials and pharmacologically active ligands. One such architecture, the β-peptoids (N-alkyl-β-alanines), has found use in a variety of biologically active compounds but has been sparsely studied with respect to
Janneke G F Hogervorst et al.
Critical reviews in toxicology, 40(6), 485-512 (2010-02-23)
Since 2002, it is known that the probable human carcinogen acrylamide is present in commonly consumed carbohydrate-rich foods, such as French fries and potato chips. In this review, the authors discuss the body of evidence on acrylamide carcinogenicity from both
Janneke G F Hogervorst et al.
Carcinogenesis, 35(5), 1032-1038 (2014-01-09)
Acrylamide, a probable human carcinogen, is present in heat-treated carbohydrate-rich foods. Epidemiological studies have not shown a clear association between acrylamide intake and colorectal cancer (CRC) risk. This may be due to the molecular heterogeneity in colorectal tumors, which was
View All Related Papers

Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

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