This product has been discontinued and is no longer available. Other lysozyme products are available and may be tested for purity as well as activity. The information available would be product dependent.
62971
Lysozyme from chicken egg white
powder (crystalline), 70000-140000 U/mg
Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
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10 G
€92.20
50 G
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.22
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biological source
chicken egg white
Quality Level
form
powder (crystalline)
specific activity
70000-140000 U/mg
mol wt
single-chain 14.3 kDa
Mr ~14600
technique(s)
cell based assay: suitable
suitability
suitable for cell lysis
UniProt accession no.
application(s)
cell analysis
storage temp.
2-8°C
Gene Information
chicken ... LYZ(396218)
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1 of 4
This Item | 62970 | L6876 | L3790 |
|---|---|---|---|
| technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable | technique(s) cell based assay: suitable |
| biological source chicken egg white | biological source chicken egg white | biological source chicken egg white | biological source chicken egg white |
| Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) | Gene Information chicken ... LYZ(396218) |
| specific activity 70000-140000 U/mg | specific activity ~100000 U/mg | specific activity ≥39,000 units/mg protein | specific activity - |
| suitability suitable for cell lysis | suitability suitable for cell lysis | suitability suitable for cell lysis | suitability suitable for cell lysis |
| application(s) cell analysis | application(s) cell analysis | application(s) cell analysis | application(s) cell analysis |
Application
Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Biochem/physiol Actions
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.
Other Notes
1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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From which buffer was the product lyophilized?
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This product is purified by ion-exchange and dialyzed to remove salt. It is 3X crystallized first in KCl and then in NaCl in its final stage.
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