Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. For more details on Bovine Serum Albumin, kindly review the following link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/263/644/a0281pis.pdf
A7517
Bovine Serum Albumin
For use as a marker in SDS-PAGE
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
Skip To
biological source
bovine
Quality Level
Assay
24-32 mg/vial (biuret)
form
lyophilized powder
mol wt
~66 kDa
purified by
cold ethanol fractionation
packaging
vial of 25 mg
origin
USA origin
concentration
24—32 mg/vial protein (biuret)
technique(s)
electrophoresis: suitable
pH
5.2
solubility
water: slightly soluble
suitability
suitable for (Suitable for use as an electrophoresis marker)
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | A2153 | A3156 | 2905-OP |
|---|---|---|---|
| assay 24-32 mg/vial (biuret) | assay ≥96% (agarose gel electrophoresis) | assay ≥99% (agarose gel electrophoresis) | assay ≥99±% (electrophoresis) |
| technique(s) electrophoresis: suitable | technique(s) ELISA: suitable, blood typing: suitable, microbiological culture: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| origin USA origin | origin USA origin | origin USA origin | origin - |
| biological source bovine | biological source bovine | biological source bovine | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| suitability suitable for (Suitable for use as an electrophoresis marker) | suitability - | suitability - | suitability - |
General description
Application
Bovine Serum Albumin (BSA) has been used:
- as a standard protein in the elution-filter aided proteome preparation (FASP) method[4] and in sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)[4][5]
- as a component of the molecular weight marker in SDS-PAGE and western blotting[5]
- as a blocking agent in immunofluorescence[5]
Biochem/physiol Actions
Bovine Serum Albumin (BSA) participates in the transport of endogenous and exogenous substances, such as hormones, fatty acids, drugs, and xenobiotics.[1] It is used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).[6] BSA is usually preferred for the preparation of serum-free media.[7] It is used in the pharmaceutical and cosmetic industry.[3]
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Preparation Note
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Structural changes during the unfolding of Bovine serum albumin in the presence of urea: A small-angle neutron scattering study
Pramana, 63(2), 363-368 (2004)
Feiran Hao et al.
The Analyst, 141(16), 4953-4960 (2016-06-17)
Currently, the separation targets of preparative electrophoresis range from milligrams to micrograms of proteins. However, most commercially available preparative electrophoretic instruments function at the milligram level. Although some preparative electrophoretic apparatuses operated at the microgram level, the fractionation results are
Mehdi Fazlalipour et al.
Jundishapur journal of microbiology, 8(4), e17157-e17157 (2015-06-03)
Gradual development of a useful vaccine can be the main point in the control and eradication of Hepatitis C virus (HCV) infection. Hepatitis C Virus envelope glycoproteins are considered as the main HCV vaccine candidate. In this study, the Pichia
Subhajit Ghosh et al.
The journal of physical chemistry. B, 119(25), 7804-7815 (2015-05-30)
The study of protein-surfactant interactions is important because of the widespread use of surfactants in industry, medicine, and pharmaceutical fields. Sodium N-lauroylsarcosinate (SL-Sar) is a widely used surfactant in cosmetics, shampoos. In this paper, we studied the interactions of bovine
Yuhong Xiao et al.
Journal of immunological methods, 384(1-2), 148-151 (2012-06-27)
The enzyme-linked immunosorbent assay (ELISA) is an extremely common and powerful laboratory technique for detecting proteins by antibodies. Researchers frequently use bovine serum albumin (BSA) as a blocking agent to prevent non-specific binding of antigens and antibodies to the microtiter
Related Content
-
how to reconstitute it.
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service