A9056

Bovine Serum Albumin

heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)

• Synonym(s): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• UNSPSC Code: 12352202
• EC Number: 232-936-2
• NACRES: NA.27
• MDL number: MFCD00130384

Properties

• Product Name: Bovine Serum Albumin, heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)
• biological source: bovine
• assay: ≥96% (agarose gel electrophoresis)
• form: lyophilized powder
• mol wt: ~66 kDa
• purified by: heat shock fractionation
• packaging: poly bottle of
• origin: USA origin
• technique(s): cell culture | mammalian: suitable
• impurities: ≤5.0% Loss on drying
• loss: ≤5%
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt accession no.: P02769
• foreign activity: BT virus, none detected; Microbiological test (pass); VSV virus, none detected; growth of Borrelia burgdorferi in BSK II medium (Suitable for)
• storage temp.: 2-8°C
• Quality Level: 200
• Gene Information: bovine ... ALB(280717)

Description

Application

Bovine Serum Albumin has been used as a supplement in Dulbecco′s modified eagle medium (DMEM).[1] It has also been used in immunostaining of cell arrays.[2]

Suitable for growth of Borrelia burgdorferi in BSK II medium

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

General description

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia.[3] Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.[4]

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)