β-Glucuronidase from Helix pomatia can be stored at –20 °C. There is no expiration date or recommended retest date on the Certificate of Analysis, however the data sheet states when stored at –20 °C, states this enzyme retains activity for at least 3 years.
G0751
β-Glucuronidase from Helix pomatia
Type H-1, partially purified powder, ≥300,000 units/g solid
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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type
Type H-1
Quality Level
form
partially purified powder
specific activity
≥300,000 units/g solid
secondary activity
≥10,000 units/g solid sulfatase
storage temp.
−20°C
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This Item | G8885 | G0762 | G8162 |
|---|---|---|---|
| specific activity ≥300,000 units/g solid | specific activity ≥90,000 units/mL | specific activity ≥60,000 units/mL | specific activity ≥5,000,000 units/g protein, pH 6.8 (biuret) |
| form partially purified powder | form aqueous solution | form aqueous solution | form aqueous glycerol solution |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| type Type H-1 | type Type H-3 | type Type H-3AF | type - |
| secondary activity ≥10,000 units/g solid sulfatase | secondary activity ≤1,000 units/mL sulfatase | secondary activity - | secondary activity - |
General description
β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Biochem/physiol Actions
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Many β-glucuronidases derived from mollusks also contain sulfatase activity.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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D Briskey et al.
European journal of nutrition, 58(5), 2087-2097 (2018-07-06)
Curcumin has been shown to deliver protective effects against numerous degenerative conditions associated with high levels of inflammation and oxidative stress. Owing to its poor bioavailability when delivered orally, it is difficult to deliver a high concentration therapeutic dose. LipiSperse®
M Richelle et al.
European journal of clinical nutrition, 53(1), 22-26 (1999-02-27)
To evaluate the plasma kinetics in man of epicatechin from black chocolate. An intervention study with 8 volunteers. Each served as his own control. Theobromine was used as control marker of the chocolate intake. Metabolic Unit, Nestle Research Center, Vers-chez-les-Blanc
Myriam Richelle et al.
The Journal of nutrition, 132(9), 2587-2592 (2002-09-11)
We investigated whether the bioavailability of isoflavones could be enhanced by enzymatic hydrolysis of glycosides to aglycones before consumption of a nonfermented soy food. Two drinks were formulated with an enriched isoflavone extract from soy germ (Fujiflavone P10), one of
Kilia Y Liu et al.
The Journal of nutrition, 150(2), 222-230 (2019-09-10)
Vitamin E α-, γ-, or δ-tocopherol (αT, γT, δT) and γ- or δ-tocotrienol (γTE, δTE) are metabolized to hydroxychromanols and carboxychromanols including 13'-carboxychromanol (13'-COOH), 11'-COOH, and carboxyethyl hydroxychroman (CEHC), some of which have unique bioactivities compared with the vitamers. However
Shu-Shu Zhong et al.
ACS omega, 6(12), 8163-8170 (2021-04-06)
Arylsulfatase and β-glucuronidase are two important enzymes in humans, which play an important role in the dynamic equilibrium of steroidal estrogens. This work probably for the first time reported that hydrogen peroxide (H2O2), hypochlorite, and peracetic acid (PAA) could effectively
Articles
β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.
Protocols
Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.
Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.
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The specification of this product is 300,000 units/g solid. The specific unit value will be determined for each lot of product and reported on the lot-specific CofA document.
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