H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

• Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• UNSPSC Code: 12161704
• PubChem Substance ID: 24895731
• NACRES: NA.75

Properties

• product line: BioXtra
• Quality Level: 400
• Assay: ≥99.5% (titration)
• form: powder
• technique(s): cell culture | embryo: suitable
• pH: 5.0-6.5 (25 °C, 238 g/L)
• useful pH range: 6.8-8.2
• pKa (25 °C): 7.5; 7.5
• solubility: water: 500 mg/mL, clear, colorless
• absorption: ≤0.05 at 290 in H₂O at 33%
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[2] It is typically used in cell culture at concentration between 5mM to 30mM.

Useful pH range: 6.8 - 8.2

Application

HEPES has been used:

• As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells[3]
• As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production[4]
• in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation[5]

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

This product has been qualified for use in cell and mouse embryo culture.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves