The product MAK106 uses linear regression fitting. The standard curve is prepared based on the donor particle concentration indicated on the label, which is batch-specific. The standard curve in table 2 is representative and for example purposes only. It's important to note that the concentrations may vary. The pmole values are calculated through dimension analysis using the amount of donor particle solution used and any subsequent dilution. For example, the most concentrated standard can be calculated by considering the volume of the donor particle. In this case, the most concentrated standard has 130 pmoles. Each subsequent dilution is two times less concentrated, resulting in concentrations of 65, 32.5, 16.25, and 8.1 pmoles.
MAK106
CETP Activity Assay Kit
Supplied by Roar Biomedical, Inc.
Synonym(s):
Cholesteryl ester transfer protein activity assay kit
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1 KIT
€720.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
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usage
sufficient for 100 fluorometric tests
application(s)
pharmaceutical
detection method
fluorometric
relevant disease(s)
cardiovascular diseases
storage temp.
2-8°C
Gene Information
human ... CETP(1071)
1 of 4
This Item | MAK305 | MAK108 | MAK110 |
|---|---|---|---|
| Gene Information human ... CETP(1071) | Gene Information - | Gene Information human ... PLTP(5360) | Gene Information human ... MTTP(4547) |
| usage sufficient for 100 fluorometric tests | usage sufficient for 100 fluorometric tests | usage sufficient for 100 fluorometric tests | usage sufficient for 100 fluorometric tests |
| detection method fluorometric | detection method fluorometric | detection method fluorometric | detection method fluorometric |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| application(s) pharmaceutical | application(s) pharmaceutical | application(s) pharmaceutical | application(s) pharmaceutical |
| relevant disease(s) cardiovascular diseases | relevant disease(s) cardiovascular diseases | relevant disease(s) cardiovascular diseases | relevant disease(s) cardiovascular diseases |
General description
Cholesteryl ester transfer protein (CETP) is present in normal human plasma. The protein transfers neutral lipids from high density lipoproteins (HDL) to very low density lipoprotein (VLDL) and low density lipoprotein (LDL). CETP plays an important role in lipoprotein metabolism and influences the reverse cholesterol transport pathway. The method is useful for measuring CETP activity in plasma or serum in all species that express CETP.
Application
Cholesteryl ester transfer protein (CETP) activity Assay Kit has been used for measurement of CETP activity in the plasma samples.[1]
Suitable for the detection of cholesteryl ester transfer protein (CETP) in plasma / serum (fresh or frozen), recombinant or purified CETP.
Biochem/physiol Actions
The CETP Activity Assay Kit uses a proprietary substrate that enables the detection of CETP-mediated transfer of neutral lipid from the substrate to a physiological acceptor. The transfer activity results in an increase in fluorescence intensity (λEx=535 nm/λEm=587 nm). In a total volume of 200 μL, the assay is linear from 0.2 to 0.8 μL of normal human plasma. Assay results not affected by endogenous plasma HDL, LDL or VLDL concentrations.
Features and Benefits
Compatible with high-throughput handling systems.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
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Tatjana Josefs et al.
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[Figure: see text].
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American journal of respiratory and critical care medicine, 199(7), 854-862 (2018-10-16)
High-density lipoprotein (HDL) cholesterol (HDL-C) levels decline during sepsis, and lower levels are associated with worse survival. However, the genetic mechanisms underlying changes in HDL-C during sepsis, and whether the relationship with survival is causative, are largely unknown. We hypothesized
Chunxiu Hu et al.
PloS one, 6(5), e19423-e19423 (2011-05-26)
Lipids are known to play crucial roles in the development of life-style related risk factors such as obesity, dyslipoproteinemia, hypertension and diabetes. The first selective cannabinoid-1 receptor blocker rimonabant, an anorectic anti-obesity drug, was frequently used in conjunction with diet
Esther M M Ooi et al.
The Journal of clinical endocrinology and metabolism, 96(10), E1568-E1576 (2011-08-06)
Dyslipidemia increases the risk of cardiovascular disease in obesity. Peroxisome proliferator-activated receptor (PPAR)-δ agonists decrease plasma triglycerides and increase high-density lipoprotein (HDL)-cholesterol in humans. The aim of the study was to examine the effect of GW501516, a PPAR-δ agonist, on
Marie Cecilie Paasche Roland et al.
Scientific reports, 11(1), 6213-6213 (2021-03-20)
Cholesteryl ester transfer protein (CETP) regulates high density lipoproteins (HDL)-cholesterol (C) and HDL-C is essential for fetal development. We hypothesized that women giving birth to large-for-gestational-age (LGA) and small-for-gestational age (SGA) infants differed in longitudinal changes in lipoproteins, CETP activity
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Instructions
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What type of regression fitting is used for the product MAK106, and what is the method for calculating the pmoles shown in the typical standard curve in table 2?
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