If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
P5368
Phosphate buffered saline
powder, pH 7.4, BioPerformance Certified, for molecular biology
Synonym(s):
PBS
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Product Name
Phosphate buffered saline, BioPerformance Certified, pH 7.4
SMILES string
OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]
InChI
InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5
InChI key
LOKCTEFSRHRXRJ-UHFFFAOYSA-I
grade
BioPerformance Certified
Molecular Biology
form
powder
storage condition
(Keep container tightly closed in a dry and well-ventilated place.)
technique(s)
immunofluorescence: suitable
impurities
BioBurden, tested
endotoxin, tested
color
white
pH
7.4
solubility
water: 9.07-10.0 g/L, clear, colorless
cation traces
Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm
suitability
suitable for SDS-PAGE
suitable for molecular biology
application(s)
diagnostic assay manufacturing
histology
sample preparation
foreign activity
DNase, RNase, Protease, none detected
Quality Level
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1 of 4
This Item | P3744 | P3813 | P38135 |
|---|---|---|---|
| technique(s) immunofluorescence: suitable | technique(s) - | technique(s) - | technique(s) - |
| grade BioPerformance Certified, for molecular biology | grade - | grade - | grade - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| form powder | form powder | form powder | form powder |
| storage condition (Keep container tightly closed in a dry and well-ventilated place.) | storage condition - | storage condition dry at room temperature | storage condition - |
Application
- Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).
- Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).
- Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).
Other Notes
Packaging
Preparation Note
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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What is the shelf-life of product P5368, phosphate buffered saline (PBS)?
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?
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No. To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?
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When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.
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Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?
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No. The complete formulation is shown in the product listing. When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.
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