Stripping and re-probing a Western blot enables sample conservation and fast correction or adjustments of antibody concentration and incubation time. This recipe is for a Western blot stripping solution that uses the surfactant sodium dodecyl sulfate (SDS) and the reducing agent β-mercaptoethanol (BME) to remove antibodies and chemiluminescent or fluorescent detection reagents.
This calculator enables the accurate preparation of this detergent-based Western blot stripping solution for any milliliter volume. Input your desired volume, click the CALCULATE button, and the table will populate with the amounts of each component needed.
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