05-1087

Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2

Name: Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
Brand: MM
Price: 687 CAD

clone 24.6.2, from mouse

Synonyme(s) :

insulin receptor substrate 1

Slide 1 of 2

Se connecter pour consulter vos tarifs contractuels et ceux de votre entreprise/organisme

Sélectionner une taille de conditionnement

100 μG

687,00 $

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.

Demander une commande groupée

A propos de cet article

Code UNSPSC :

12352203

eCl@ss :

32160702

Nomenclature NACRES :

NA.41

Principaux documents

Voir toute la documentation

Passer à

Service technique

Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.

Laissez-nous vous aider

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified antibody

Type de produit anticorps

primary antibodies

Clone

24.6.2, monoclonal

Espèces réactives

bovine, pig, mouse, monkey, canine, rat, human

Technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

NP_005535.1

Numéro d'accès UniProt

P35568

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pSer307 )

Informations sur le gène

bovine ... Irs1(538598)
dog ... Irs1(486148)
human ... IRS1(3667)
mouse ... Irs1(16367)
pig ... Irs1(100512686)
rat ... Irs1(25467)
rhesus monkey ... Irs1(707870)

Comparer avec des articles similaires

Voir la comparaison complète

Montrer les différences

1 of 4

Cet article	05-1086	05-1085	05-784R
Sigma-Aldrich 05-1087 Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2	Sigma-Aldrich 05-1086 Anti-phospho-IRS1 (Ser302 mouse/ Ser307 human) Antibody, clone 8.1.2	Sigma-Aldrich 05-1085 Anti-IRS1 Antibody, clone 4.2.2	Sigma-Aldrich 05-784R Anti-IRS1 Antibody, clone 58-10C-31, rabbit monoclonal
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
clone 24.6.2, monoclonal	clone 8.1.2, monoclonal	clone 4.2.2, monoclonal	clone 58-10C-31, monoclonal
species reactivity bovine, pig, mouse, monkey, canine, rat, human	species reactivity bovine, pig, canine, monkey, human, rat, mouse	species reactivity pig, canine, human, mouse, rat, monkey, bovine	species reactivity human, rat, mouse
antibody form purified antibody	antibody form purified antibody	antibody form purified antibody	antibody form purified immunoglobulin
UniProt accession no. P35568	UniProt accession no. P35568	UniProt accession no. P35568	UniProt accession no. P35570
technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunofluorescence: suitable, western blot: suitable	technique(s) immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) immunoprecipitation (IP): suitable, western blot: suitable

Description générale

IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.

Env. 185 kDa

Immunogène

Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.

Application

This Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 is validated for use in WB, IP, IC, IF for the detection of phospho-IRS1 (Ser307 mouse/ Ser312 human).

Actions biochimiques/physiologiques

Predicted to cross-react with many other species based on 100% sequence homology with immunogen.

This antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human).

Forme physique

Format : Produit purifié

Remarque sur l'analyse

1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.

Autres remarques

Concentration : pour connaître la concentration spécifique du lot, voir le certificat d'analyse.

Remplace le(s) produit(s) suivant(s) : 07-247

Vous ne trouvez pas le bon produit ?

Essayez notre Outil de sélection de produits.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Nicotinamide Mononucleotide Prevents Free Fatty Acid-Induced Reduction in Glucose Tolerance by Decreasing Insulin Clearance.

Ashraf Nahle et al.

International journal of molecular sciences, 22(24) (2021-12-25)

The NAD-dependent deacetylase SIRT1 improves β cell function. Accordingly, nicotinamide mononucleotide (NMN), the product of the rate-limiting step in NAD synthesis, prevents β cell dysfunction and glucose intolerance in mice fed a high-fat diet. The current study was performed to

MicroRNA-181b Improves Glucose Homeostasis and Insulin Sensitivity by Regulating Endothelial Function in White Adipose Tissue.

Xinghui Sun et al.

Circulation research, 118(5), 810-821 (2016-02-03)

The pathogenesis of insulin resistance involves dysregulated gene expression and function in multiple cell types, including endothelial cells (ECs). Post-transcriptional mechanisms such as microRNA-mediated regulation of gene expression could affect insulin action by modulating EC function. To determine whether microRNA-181b

Inhibition of JNK suppresses autophagy and attenuates insulin resistance in a rat model of nonalcoholic fatty liver disease.

Hua Yan et al.

Molecular medicine reports, 15(1), 180-186 (2016-12-03)

Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, the pathological process of which is complex. Activation of the c‑Jun N‑terminal kinase (JNK) signaling pathway is associated with the mechanism underlying obesity-induced insulin resistance. Furthermore, the JNK signaling

Aldosterone Jeopardizes Myocardial Insulin and β-Adrenergic Receptor Signaling via G Protein-Coupled Receptor Kinase 2.

Alessandro Cannavo et al.

Frontiers in pharmacology, 10, 888-888 (2019-08-27)

Hyperaldosteronism alters cardiac function, inducing adverse left ventricle (LV) remodeling either via increased fibrosis deposition, mitochondrial dysfunction, or both. These harmful effects are due, at least in part, to the activation of the G protein-coupled receptor kinase 2 (GRK2). In

Inhibition of Protein Tyrosine Phosphatase 1B Improves IGF-I Receptor Signaling and Protects Against Inflammation-Induced Gliosis in the Retina.

Ana I Arroba et al.

Investigative ophthalmology & visual science, 56(13), 8031-8044 (2016-01-01)

Insulin-like growth factor-I receptor (IGF-IR) signaling mediates retinal growth and survival and its failure may contribute to aggravate diabetic retinopathy (DR). Protein tyrosine phosphatase 1B (PTP1B) negatively modulates IGF-IR signaling, but its involvement in inflammation during DR remains unknown. We

Afficher tous les articles scientifiques apparentés

Questions

Reviews

No rating value

Active Filters

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique