ABE1322

Anti-MBD5

from rabbit, purified by affinity chromatography

• Synonyme(s) : Methyl-CpG-binding domain protein 5, Methyl-CpG-binding protein MBD
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.43

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), mouse (based on 100% sequence homology)
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_060798.2
• Numéro d'accès UniProt: Q9P267
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... MBD5(55777)

Description

Description générale

Methyl-CpG-binding domain protein 5 (UniProt: Q9P267; also known as Methyl-CpG-binding protein MBD5) is encoded by the MBD5 (also known as KIAA1461) gene (Gene ID: 55777) in human. MBD proteins play a role in diverse processes, including chromatin remodeling, DNA damage repair, histone methylation, and X chromosome inactivation. MBD5 is detected in heart, placenta, liver, skeletal muscle, kidney, and pancreas. It binds to heterochromatin, but does not interact with either methylated or unmethylated DNA (in vitro). It contains a MBD domain (aa 11-81) and a PWWP (Pro-Trp-Trp-Pro) domain (aa 1385-1447). Both MBD and PWWP domains are shown to be essential for chromocentric localization. Two isoforms of MBD5 have been reported that are produced by alternative splicing. However, only the isoform 1 associates with pericentric heterochromatin. Mutations in MBD5 gene are known to cause autosomal dominant mental retardation, which is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. (Ref.: Camarena, V et al., (2014). EMBO Mol. Med. 6(8); 1003 1015).

~160 kDa observed; 159.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogène

Epitope: unknown

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Methyl-CpG-binding domain protein 5 (MBD5).

Application

Anti-MBD5, Cat. No. ABE1322, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD5 in human pancreas and human cervical cancer tissue sections

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD5 in HeLa, A431, and HEK293 cells.

Western Blotting Analysis: 2 µg/mL from a representative lot detected MBD5 in human liver tissue lysate.

Research Category
Epigenetics & Nuclear Function

Actions biochimiques/physiologiques

This rabbit polyclonal antibody detects isoform 1 of human Methyl-CpG-binding domain protein 5. It targets an epitope within 16 amino acids fromt the C-terminal region.

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Notes préparatoires

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Evaluated by Immunohistochemistry in human prostate tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MBD5 in human prostate tissue sections.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.