08445

7-Amino-4-methyl-3-coumarinylacetic acid

BioReagent, suitable for fluorescence, ~90% (HPLC)

• Synonyme(s) : 2H-1-Benzopyran-3-acetic acid, AMCA-H, Aminomethyl coumarin acetic acid
• Formule empirique (notation de Hill) : C₁₂H₁₁NO₄
• Numéro CAS: 106562-32-7
• Poids moléculaire : 233.22
• NACRES: NA.32
• PubChem Substance ID: 329748585
• UNSPSC Code: 12352106
• MDL number: MFCD00081051
• Beilstein/REAXYS Number: 7927205
• eCl@ss: 32150319

Propriétés

• product line: BioReagent
• Quality Segment: 100
• assay: ~90% (HPLC)
• form: powder
• solubility: DMF: soluble, DMSO: soluble, aqueous base: soluble
• fluorescence: λ_ex 350 nm; λ_em 433 nm in methanol
• suitability: suitable for fluorescence
• SMILES string: CC1=C(CC(O)=O)C(=O)Oc2cc(N)ccc12
• InChI: 1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15)
• InChI key: QEQDLKUMPUDNPG-UHFFFAOYSA-N

Description

Application

AMCA is a useful agent for labeling proteins. Demonstrates a characteristic absorption peak at 345 nm with a fluorescence emission at 440-460 nm (blue region); however, when conjugated with proteins the absorption peak shifts to 355 nm with a fluorescence emission at 440-460 nm. It has a high fluorescence yield with a sufficient Stokes shift (100 nm) and is relatively photostable . Relatively easy to activate and couple to the N-terminal amino group of a peptide resulting in an acid stable amide bond .

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Useful blue fluorophore for immunofluorescence, λ_abs ~350 nm, λ_em ~440 nm

Informations sur la sécurité

• Pictogrammes: GHS07
• Mot-clé: Warning
• Codes de danger: H315,H319,H335
• Mentions de précaution: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• Organes cibles: Respiratory system
• Classe de stockage: 11 - Combustible Solids
• WGK: WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• EPI (équipement de protection individuelle): dust mask type N95 (US), Eyeshields, Gloves