This product is a conjugate of 5'-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5'-ATP. A 9-atom matrix spacer arm connects the ATP and the agarose. Please see the link below to review the product datasheet for more information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/174/887/a2767pis.pdf
A2767
Adenosine 5′-triphosphate–Agarose
lyophilized powder
Synonyme(s) :
5′-ATP agarose
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1 ML
252,00 $
5 ML
968,00 $
25 ML
3 880,00 $
252,00 $
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Laissez-nous vous aiderSource biologique
plant (Sea weed)
Niveau de qualité
Forme
lyophilized powder
Ampleur du marquage
1-5 μmol per mL
Matrice
cross-linked 4% beaded agarose
Activation de la matrice
cyanogen bromide
Fixation de matrice
C-8
Espaceur de matrice
9 atoms
Température de stockage
−20°C
Chaîne SMILES
[X]Nc1ncnc2[n](cnc21)[C@@H]3O[C@@H]([C@H]([C@H]3O)O)CO[P](=O)(O[P](=O)(O[P](=O)(O)O)O)O
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Catégories apparentées
1 of 4
Cet article | A6888 | A2810 | 02065 |
|---|---|---|---|
| biological source plant (Sea weed) | biological source - | biological source - | biological source - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form aqueous glycerol suspension | form lyophilized powder | form powder (lyophilized) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| extent of labeling 1-5 μmol per mL | extent of labeling ≥1 μmol per mL | extent of labeling 1-5 μmol per mL | extent of labeling - |
| matrix cross-linked 4% beaded agarose | matrix cross-linked 4% beaded agarose | matrix cross-linked 4% beaded agarose | matrix - |
Description générale
Adenosine 5′-triphosphate–Agarose (5′-ATP-agarose) is a conjugate of 5′-ATP to crosslinked 4% beaded agarose (activated by cyanogen bromide), via the C-8 atom of 5′-ATP. 5′-ATP–agarose is applicable for affinity purification of various proteins and enzymes like cyclin-dependent kinase 2 (CDK2), heat shock proteins, and cryptochromes.
Application
Adenosine 5′-triphosphate Agarose (5′-ATP agarose) has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.
Adenosine 5′-triphosphate–Agarose (5′-ATP–agarose) is applicable for affinity purification of various proteins and enzymes like cyclin-dependent kinase 2 (CDK2), heat shock proteins-70 (HSP-70), and cryptochromes. 5′-ATP agarose has been used in affinity chromatography to purify uridine kinase from Ehrlich ascites tumor cells.
Forme physique
Lyophilized powder stabilized with lactose
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
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J E Braun et al.
The Journal of biological chemistry, 271(42), 25989-25993 (1996-10-18)
Cysteine string protein (CSP) is a 34 kDa secretory vesicle protein bearing a "J-domain" as well as a palmitoylated cysteine-rich "string" region used for membrane attachment. Mutation of the CSP gene causes impaired presynaptic neuromuscular transmission in Drosophila melanogaster, implicating
M Brungs et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(1), 107-111 (1995-01-03)
5-Lipoxygenase (5-LO; EC 1.13.11.34) activity in the human monocytic cell line Mono Mac 6 was upregulated by combined treatment with transforming growth factor beta 1 (TGF-beta) and 1,25-dihydroxyvitamin D3 (VD3). In undifferentiated cells, 5-LO enzyme activity was undetectable. After the
Christian Feisst et al.
The Journal of pharmacology and experimental therapeutics, 315(1), 389-396 (2005-07-15)
Myrtucommulone (MC) and semimyrtucommulone (S-MC) are unique oligomeric, nonprenylated acylphloroglucinols contained in the leaves of myrtle (Myrtus communis). Although extracts of myrtle have been traditionally used in folk medicine for the treatment of various disorders, studies addressing select cellular or
C Prodromou et al.
The EMBO journal, 18(3), 754-762 (1999-02-02)
The in vivo function of the heat shock protein 90 (Hsp90) molecular chaperone is dependent on the binding and hydrolysis of ATP, and on interactions with a variety of co-chaperones containing tetratricopeptide repeat (TPR) domains. We have now analysed the
Erin E Nicklow et al.
The Journal of biological chemistry, 295(2), 552-569 (2019-12-07)
Cells employ a vast network of regulatory pathways to manage intracellular levels of reactive oxygen species (ROS). An effectual means used by cells to control these regulatory systems are sulfur-based redox switches, which consist of protein cysteine or methionine residues
Contenu apparenté
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Where is the linker attached on the ATP molecule
1 answer-
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Can I store Product A2767, Adenosine 5´-triphosphate-Agarose, after I have hydrated it?
1 answer-
For the short term, it is possible to store the hydrated resin in 20% ethanol and dilute buffer at 2-8°C. In general, the hydrated resin can be stored refrigerated in water or buffer containing a bacteriostat, such as 0.02% sodium azide or thimerosal. Do not autoclave or freeze the hydrated resin. The resin can be used several times without loss of effectiveness. However, the ATP will slowly hydrolyze to ADP over time, and under certain conditions this process may be accelerated during usage. The resin can be regenerated; please see the regeneration FAQ for this product.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I regenerate Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
Since N6-[N-(6-aminohexyl)-carbamyl]-ADP is a substrate for acetate kinase and pyruvate kinase, these enzymes can be used to regenerate ATP from ADP on the resin under conditions favoring the reverse reaction. Wash the resin with 20 volumes of 50 mM Tris HCl buffer (pH 8.2), with 0.2 mM EDTA and 100 mM KCl. Incubate the resin overnight at 2-8°C in an ATP-regenerating mixture containing 0.2 mM phosphoenol pyruvate, pyruvate kinase (10 units per mL of resin), 5 mM MgCl2, 0.2 mM EDTA and 100 mM KCl in 50 mM Tris HCl buffer (pH 8.2). Wash the resin with 25 column volumes of 2 mM ATP in 2 M KCl and reequilibrate the resin with 25 column volumes of sample buffer. Alternatively, the ATP regenerating mixture can contain 20 mM acetyl phosphate, acetate kinase (6.8 units per mL of resin), and 3 mM MgCl2 in 100 mM Tris HCl (pH 7.6). If acetate kinase is used, prewash the resin with 25 column volumes of 100 mM Tris HCl (pH 7.6), but use the same final wash and reequilibration steps as given for pyruvate kinase.
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How can I elute my proteins from Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
Specifically bound proteins can be eluted with 10-100 mM ATP or ADP. Nonspecifically bound proteins can be eluted with 2 M NaCl or KCl in water or 7 M urea.
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How can I hydrate Product A2767, Adenosine 5´-triphosphate-Agarose?
1 answer-
The resin can be hydrated by placing it in excess water, approximately 50 mL per g of resin, for at least 30 minutes. Remove the lactose stabilizer by washing the resin on a Buchner funnel with gentle vacuum, using approximately 100 mL of water per g of resin. Do not allow the resin to dry. Resuspend the resin in excess water or starting buffer to pack the column bed.
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