As mentioned in the Preparation Note on the product webpage, the collagen is "prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
The presence of Atelo or Telo collagen is not determined.
C7774
Human Collagen Type I
from human placenta, powder, suitable for cell culture
Se connecter pour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
5 MG
261,00 $
25 MG
1 090,00 $
261,00 $
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
A propos de cet article
Numéro CAS:
Numéro CE :
Numéro MDL:
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.61
Passer à
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
Collagène from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
Source biologique
human placenta
Forme
powder
Technique(s)
cell culture | mammalian: suitable
Impuretés
HIV, hepatitis B and hepatitis C, none detected
Température de stockage
2-8°C
Informations sur le gène
bovine ... CSN2(281099), CSN3(281728)
human ... COL4A3(1285), COL4A4(1286), COL4A6(1288)
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
1 of 4
Cet article | C4407 | C5608 | C5483 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable |
| biological source human placenta | biological source human placenta | biological source rabbit skin | biological source human |
| form powder | form powder | form powder | form powder |
| Gene Information bovine ... CSN2(281099), CSN3(281728) | Gene Information human ... COL3A1(1300) | Gene Information rabbit ... COL1A2(100008997) | Gene Information human ... COL1A2(1278) |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| impurities HIV, hepatitis B and hepatitis C, none detected | impurities HIV, hepatitis B and hepatitis C, none detected | impurities - | impurities - |
Application
This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm2. It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.
Actions biochimiques/physiologiques
Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.
Notes préparatoires
Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.
Autres remarques
Le collagène se classe en un certain nombre de types structurellement et génétiquement distincts. Nous utilisons la nomenclature proposée par Bornstein et Traub. Il est important de ne pas confondre les désignations utilisées par Sigma et les types de classification reconnus.
All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Marta Biedzka-Sarek et al.
Infection and immunity, 76(11), 5016-5027 (2008-09-04)
Yersinia enterocolitica is an enteric pathogen that exploits diverse means to survive in the human host. Upon Y. enterocolitica entry into the human host, bacteria sense and respond to variety of signals, one of which is the temperature. Temperature in
Yinglin Feng et al.
Frontiers in endocrinology, 12, 664766-664766 (2021-08-24)
Preeclampsia is a common obstetric disorder affecting 2-8% of pregnancy worldwide. Fibrosis is an important histological change occurring in preeclamptic placenta, and might depend on the excess deposition of collagen I. However, the role of fibrotic placenta and collagen I
Riina Harjumäki et al.
Scientific reports, 9(1), 7354-7354 (2019-05-16)
In vitro cell culture or tissue models that mimic in vivo cellular response have potential in tissue engineering and regenerative medicine, and are a more economical and accurate option for drug toxicity tests than animal experimentation. The design of in
Ria Aryani Hayuningtyas et al.
Molecular medicine (Cambridge, Mass.), 27(1), 125-125 (2021-10-05)
C1q has been reported to reveal complement-independent roles in immune and non-immune cells. C1q binds to its specific receptors to regulate distinct functions that rely on the environment and cell types. Discoidin domain receptor 2 (DDR2) is activated by collagen
Annika Hunziker et al.
Cell reports, 38(4), 110306-110306 (2022-01-27)
Binding of influenza virus to its receptor triggers signaling cascades that reprogram the cell for infection. To elucidate global virus-induced changes to the cellular signaling landscape, we conducted a quantitative phosphoproteomic screen with human and avian influenza viruses. Proteins with
Contenu apparenté
Instructions
-
Is Human Collagen Type I, (Cat # C7774) isolated enzymatically or by acid extraction? Atelo or Telo?
1 answer-
Helpful?
-
-
해당 제품의 순도가 궁금합니다.
1 answer-
Unfortunately, the purity of this product is not determined. An SDS-PAGE is performed and the electrophoretic profile is confirmed to be consistent with past lots. For additional information please see the link below to the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/348/610/c7774pis.pdfHelpful?
-
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique