This product is an Immunoprecipitation kit that includes both glycine and albumin in the protocol. The albumin is utilized as a blocking agent, and the glycine is used to stop the cross-linking reaction. Please see the link below to review the complete product information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/308/933/chp2bul.pdf
CHP2NC
Imprint® Ultra Chromatin Immunoprecipitation Kit, Without Controls
ChIP kit for maximum sensitivity, compatible with Next-Gen sequencing
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A propos de cet article
Code UNSPSC :
41116158
Nomenclature NACRES :
NA.54
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Description générale
The Imprint Ultra Chromatin IP Kit is Sigma′s second generation chromatin immunoprecipitation (ChIP) kit developed for maximum sensitivity and optimum next-generation sequencing results. It provides a complete solution for Chromatin Immunoprecipitation, including columns and reagents for DNA purification. The kit allows researchers to explore the genome-wide binding sites of low abundance transcription factors (TFs), as well as novel histone modifications. It is optimized for ChIP reactions with chromatin from 106 cells (up to ~50 μg DNA), and can also be scaled up (or several preparations pooled) to accommodate 108 cells for genome-wide binding studies in ChIP-chip and ChIP-Seq applications.
Application
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Caractéristiques et avantages
- Use successfully CHiP′ed DNA associated with low abundance, medium, and highly expressed transcription factors, as well as histone modifications
- Greater capacity - can be used over a wide range of cell numbers ranging from 2-10 × 106
- Maximum sensitivity - capable of detecting low abundance transcription factors with as little as 2 × 106 cells
- Employs DNA-Blocked "Staph-Seq" for IP (immunoprecipitation), minimizing contaminating Staph A DNA in downstream ChIP-Seq applications.
Informations légales
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit seuls
Réf. du produit
Description
- Sodium dodecyl sulfate solution, 10% in 18 megohm water
- Monoclonal Anti-POLR2A antibody produced in mouse, clone 1F17, purified immunoglobulin, buffered aqueous solution
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS
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- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powder
- A7638Bovine Serum Albumin, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)
- D9156Deoxyribonucleic acid, single stranded from salmon testes, For hybridization
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
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Mention d'avertissement
Danger
Mentions de danger
Classification des risques
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Organes cibles
Central nervous system
Code de la classe de stockage
5.1A - Strongly oxidizing hazardous materials
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Chromatin isolation by RNA purification (ChIRP).
Chu, C., et al.
Journal of Visualized Experiments (2012)
Comparison of sample preparation methods for ChIP-chip assays.
O'Geen, H., et al.
Biotechniques, 41, 557-557 (2006)
Philippe Collas
Molecular biotechnology, 45(1), 87-100 (2010-01-16)
The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest
Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Chu, C., et al.
Molecular Cell (2011)
Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
Protocoles
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
Contenu apparenté
Réactifs pour interactions entre protéines et acides nucléiques, ressources permettant d'étudier les interactions protéines/ARN, protéines/ADN et protéines/protéines, et applications associées.
Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.
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