DAPI is suitable for staining bacterial chromosomes, including those of E. coli.
D9542
DAPI
for nucleic acid staining
DAPI
Synonyme(s) :
4′,6-Diamidino-2-phénylindole dihydrochloride, 2-(4-Amidinophényl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
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17,70 $
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93,40 $
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602,00 $
A propos de cet article
Formule empirique (notation de Hill) :
C16H15N5 · 2HCl
Numéro CAS:
Poids moléculaire :
350.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
249-186-7
Beilstein/REAXYS Number:
4894417
MDL number:
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Laissez-nous vous aiderNom du produit
DAPI, for nucleic acid staining
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
grade
Molecular Biology
assay
≥98% (HPLC)
form
powder
technique(s)
transfection: suitable
solubility
H2O: 20 mg/mL
PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O at 1 mM
fluorescence
λex 340 nm; λem 488 nm (nur DAPI)
λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
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Catégories apparentées
1 of 4
Cet article | 10236276001 | 268298 | MBD0020 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability - | suitability - | suitability suitable for nucleic acid staining |
| grade for molecular biology | grade - | grade - | grade - |
| technique(s) transfection: suitable | technique(s) - | technique(s) - | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable |
| fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex ~359 nm, λem ~461 nm | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
| form powder | form powder | form solid | form liquid |
| solubility H2O: 20 mg/mL, PBS: insoluble | solubility - | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble | solubility - |
Application
Adapté aux applications suivantes :
Le DAPI a été utilisé :
- Coloration de l′ADN dans des gels d′agarose
- Analyse des modifications de l′ADN lors de l′apoptose
- Détection des mycoplasmes
- Photofootprinting de l′ADN
- Coloration par immunofluorescence des cellules
Le DAPI a été utilisé :
- pour la surveillance rapide de la contamination microbiologique[1]
- dans la technique de coloration des bandes de chromosomes[2]
- pour la détection des cellules apoptotiques[3]
- en microscopie à fluorescence, pour suivre le mouvement de la protéine DisA (DNA integrity scanning protein) sur l′ADN de Bacillus subtilis[4]
- pour colorer les grains de pollen matures (0,5 mg/ml)[5]
Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose.
Le DAPI est nettement plus sensible que le bromure d′éthidium pour marquer l′ADN dans les gels d′agarose. Il peut être utilisé dans le photofootprinting de l′ADN, pour détecter les sondes hybridées dans les applications sur membranes en permettant la visualisation spécifique des complexes double-brins, et pour évaluer les changements de l′ADN et de son contenu lors de l′apoptose par cytométrie en flux. La coloration par DAPI est également une méthode sensible et spécifique de détection des mycoplasmes.
Biochem/physiol Actions
Sonde fluorescente pour l′ADN qui se lie dans le petit sillon, capable de pénétrer dans les cellules. Se lie au petit sillon d′ADN double brin (préférentiellement à l′ADN riche en AT), formant un complexe stable dont l′intensité de fluorescence est environ 20 fois plus élevée que celle du DAPI non lié.
Disclaimer
Protéger de la lumière.
General description
Le DAPI (dichlorhydrate de 4′,6-Diamidino-2-phénylindole) est un colorant fluorescent capable de pénétrer dans les cellules et de se lier à l′ADN.
la chaleur ou la sonication peuvent être nécessaires. Les solutions stockées à l'abri de la lumière à température ambiante ou à 4 °C devraient être stables pendant 2 à 3 semaines.
Related product
Réf. du produit
Description
Tarif
signalword
Warning
hcodes
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
target_organs
Respiratory system
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
J Kapuscinski
Biotechnic & histochemistry : official publication of the Biological Stain Commission, 70(5), 220-233 (1995-09-01)
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. The physicochemical properties of the dye and
Christy A Itoga et al.
The Journal of comparative neurology, 527(15), 2474-2487 (2019-03-13)
Corticotropin-releasing hormone (CRH) is an essential, evolutionarily-conserved stress neuropeptide. In addition to hypothalamus, CRH is expressed in brain regions including amygdala and hippocampus where it plays crucial roles in modulating the function of circuits underlying emotion and cognition. CRH+ fibers
S Ituarte et al.
Genetica, 122(2), 199-206 (2004-12-22)
Male karyotype and meiosis of Tenagobia fuscata (Corixoidea, Micronectidae) are studied. The species possesses a male diploid chromosome number 2n = 28 + XY, holokinetic chromosomes, absence of m chromosomes and an achiasmatic male meiosis. Autosomes divide pre-reductionally while the
R Howden et al.
Genetics, 149(2), 621-631 (1998-06-11)
As a strategy for the identification of T-DNA-tagged gametophytic mutants, we have used T-DNA insertional mutagenesis based on screening for distorted segregation ratios by antibiotic selection. Screening of approximately 1000 transgenic Arabidopsis families led to the isolation of eight lines
Yan Li et al.
Toxicology letters, 154(3), 225-233 (2004-10-27)
Excessive exposure to synthetic and endogenous estrogens has been associated with the development of cancer in several tissues including the breast. 4-Hydroxyequilenin (4-OHEN), a major catechol metabolite of equine estrogens present in Premarin, an estrogen replacement formulation, has been shown
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Instructions
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Can this product be used to stain bacteria (E.coli) chromosome?
1 answer-
Helpful?
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What is the working dilution of DAPI that should be used to stain the nuclei of human cells?
1 answer-
Typical working concentration of DAPI used in most applications is 0.1μg/mL-10μg/mL. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/774/984/d9564pis.pdfPlease see the link below to review a sample protocol from Cold Spring Harbor:
https://cshprotocols.cshlp.org/content/2011/1/pdb.prot5556.longHelpful?
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what kind of the water used to dissolve DAPI? and what's the temperature should be dissolve the water?
1 answer-
This product is soluble at 20 mg/mL in water at ambient temperature. No specific water type or treatment is required. Deionized water is typically used for laboratory applications.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfHelpful?
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Can the stain concentration be used for staining fish scales and any animal cells for ICC?
1 answer-
Yes, DAPI can be used to stain fish scale nuclei. While specific data regarding the concentration and duration for staining fish scale nuclei is not available, it is suggested to start with a working concentration of around 0.5 mg/ml and optimize the staining for each specific cell type, as different cells may uptake the dye at varying rates. It is also advisable to try staining at both higher and lower concentrations. For staining mammalian cells, lower concentrations are typically used.
Helpful?
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What is the suggested concentration for product D9542-1MG?
1 answer-
The suggested concentration for product D9542-1MG, DAPI, is in the range of 0.5 to 5 ug/ml.
Helpful?
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What is the suitable DAPI for immunofluorescence staining of free-floating rat brain sections?
1 answer-
Several DAPI products are suitable for staining free-floating rat brain sections. The same DAPI used for staining mycoplasma, cultured cells, or other cell types can be used for this purpose. Any of the DAPI products listed on the website should be suitable for this use.
Helpful?
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肝細胞を染める染色プロトコルを教えてください。
1 answer-
Please see the link below to review the product datasheet, which includes basic protocols:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/392/209/d9542pis.pdfA protocol specifically for hepatocyte staining is not available. However, the publication shown at the link below may be helpful:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5671579/Helpful?
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Aside from the applications described on the product page, how is D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), used?
1 answer-
DAPI is used as a nuclear counterstain in our FISH protocol at 2 μg/mL in antifade mounting media. DAPI can also be used as a vital dye to stain mature pollen grains (0.5 μg/mL). DAPI can bind to RNA, but its selectivity for DNA over RNA is high. It appears that DAPI uses an intercalating binding mode with RNA that is AU selective (Biochem., 31, 3103 (1992)). Selectivity of DAPI for DNA over RNA is greater than that shown by ethidium bromide and propidium iodide (Nucleic Acid Research, 6, 3535 (1979))
Helpful?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be dissolved?
1 answer-
This product is soluble in water at 20 mg/mL, but may require heat. DAPI does not dissolve readily in PBS, 0.1 M phosphate, or 0.1 M Tris (pH 7).
Helpful?
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How should D9542, 4´,6-Diamidino-2-phenylindole dihydrochloride (DAPI), be stored once in solution, and for how long would it be good?
1 answer-
Solutions stored in the dark at room temperature have been stable for 2-3 weeks, but solutions exposed to light for the same time period have shown considerable degradation. At 1 mg/mL, solutions are stable in the dark at 2-8 °C for several weeks. For long-term storage, the solutions can be divided into aliquots and stored at -20 °C.
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