The protocol is published in the product datasheet, available in the 'DOCUMENTATION' section of this page, under the 'Mor Documents' tab. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf
MINI26
PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonyme(s) :
Red PKH membrane label kit
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1 KIT
470,00 $
470,00 $
Disponible dès aujourd'huiDétails
A propos de cet article
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32
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Laissez-nous vous aiderConditionnement
pkg of 1 kit
Niveau de qualité
Fabricant/nom de marque
Distributed for Phanos Technologies
Conditions de stockage
protect from light
Technique(s)
flow cytometry: suitable
Fluorescence
λex 551 nm; λem 567 nm (PKH26 dye)
Application(s)
cell analysis
detection
Méthode de détection
fluorometric
Conditions d'expédition
ambient
Température de stockage
room temp
1 of 4
Cet article | PKH26GL | MINI67 | PKH26PCL |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable | technique(s) - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - |
| manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename - |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition - |
| fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence - |
| application(s) cell analysis | application(s) - | application(s) cell analysis | application(s) - |
Description générale
This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.
Application
PKH26 red fluorescent cell linker mini kit has been used for:
Autres remarques
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Informations légales
CellVue is a registered trademark of Phanos Technologies
Composants de kit seuls
Réf. du produit
Description
- Diluent C 10 mL
- PKH26 Cell Linker in ethanol .1 mL
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
57.2 °F - closed cup
Point d'éclair (°C)
14 °C - closed cup
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
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Imaging of peripheral benzodiazepine receptor expression as biomarkers of detrimental versus beneficial glial responses in mouse models of Alzheimer's and other CNS pathologies
Ji B, et al.
The Journal of Neuroscience, 28(47), 12255-12267 (2008)
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Lee HY, et al.
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Human leukocyte antigen-G1 inhibits natural killer cytotoxicity through blocking the activating signal transduction pathway and formation of activating immunologic synapse
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Articles
Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.
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MINI26-1KTの実験プロトコルを入手したいのですが、どこにありますか？
1 answer-
Helpful?
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Is it possible to lable cells with PKH26 at 4oC?
1 answer-
The membrane dyes will remain stable during the monoclonal staining at 4 deg C; however, capping of the monoclonal antibodies is highly probable if the general cell membrane labeling is carried out at ambient temperature subsequent to antibody labeling. As additional information may be required to address the question completely, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the information needed so that a member of the Technical Service team can reach out to assist further.
For more information, please refer to the product data-sheet below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf.
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