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MilliporeSigma

P7119

PGO Enzyme Preparation

1 G capsules

Synonyme(s) :

Peroxidase - Glucose oxidase preparation

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10 CAPS

232,00 $

232,00 $


Disponible dès aujourd'huiDétails



A propos de cet article

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

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Niveau de qualité

Forme

powder

Solubilité

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

Température de stockage

2-8°C

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Cet article
345386G6125P8125
form

powder

form

lyophilized solid

form

powder

form

essentially salt-free, lyophilized powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

solubility

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

solubility

0.1 M phosphate buffer, pH 7.0: 5 mg/mL

solubility

-

solubility

0.1 M phosphate buffer: soluble (pH 6.0), H2O: soluble

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Description générale

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Application

PGO Enzymes preparation has been used:
  • in diet sampling and chemical analyses[1][2]
  • to determine plasma and follicular fluid glucose levels[3]
  • to measure glucose content in fractions of retrograded debranched rice starch[4]
Tested and found suitable for use in the quantitation of glucose.

Actions biochimiques/physiologiques

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

Notes préparatoires

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

Pictogrammes

Health hazard

Mention d'avertissement

Danger

Mentions de danger

Conseils de prudence

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Consulter la Bibliothèque de documents

R Salehi et al.
Journal of dairy science, 99(5), 3584-3597 (2016-03-14)
The objectives were to determine the effects of supplemental fat (no oilseed vs. oilseed) during late gestation and the source of fat (canola vs. sunflower seed), on dry matter intake (DMI), plasma metabolite concentrations, milk production and composition, calf birth
M T Harper et al.
Journal of dairy science, 100(8), 6151-6163 (2017-06-12)
The objective of this experiment was to partially replace corn silage with 2 alternative forages, wheat (Triticum aestivum) or triticale (X Triticosecale) silages at 10% of the diet dry matter (DM), and investigate the effects on dairy cow productivity, nutrient
Reza Salehi et al.
Reproductive biology and endocrinology : RB&E, 13, 69-69 (2015-07-04)
The objective was to determine the effect of prepartum diets supplemented with rolled canola seed (high in oleic acid) or sunflower seed (high in linoleic acid) on luteinizing hormone (LH) pulsatility and gonadotropin releasing hormone (GnRH)-induced LH release during early
P Piantoni et al.
Journal of dairy science, 98(5), 3323-3334 (2015-03-03)
Forty-eight multiparous cows were used in a randomized complete block design experiment with a 2×2 factorial arrangement of treatments to determine the interaction between a highly saturated free FA supplement (SFFA) and dietary forage NDF (fNDF) content on energy balance
Evaluation of triticale dried distillers grains with solubles as a substitute for barley grain and barley silage in feedlot finishing diets
K. T. Wierenga, T. A. McAllister, et al.
Journal of Animal Science, 88, 2018-3029 (2010)

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