Proteins cannot be transferred to a membrane after being stained and de-stained with the Product RPROB, Reversible Protein Detection Kit.
RPROB
Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels
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1 KIT
414,00 $
414,00 $
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A propos de cet article
Code UNSPSC :
12352200
Nomenclature NACRES :
NA.32
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Laissez-nous vous aiderUtilisation
sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels)
Niveau de qualité
Technique(s)
protein staining: suitable
Température de stockage
2-8°C
1 of 4
Cet article | GLYCOPRO | RSB | PROTSIL2 |
|---|---|---|---|
| usage sufficient for 20 applications staining (small 8 X 10 cm blots or PAGE gels) | usage kit sufficient for 10 mini-gels (10 × 10 cm), kit sufficient for 5 gels or membranes (16.5 × 19 cm) | usage - | usage kit sufficient for 25 mini-gels (10 × 10 cm) |
| technique(s) protein staining: suitable | technique(s) - | technique(s) protein staining: suitable | technique(s) protein staining: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 200 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 20-25°C |
Application
R-PROB is a unique protein detection system designed for staining of proteins on nylon, nitrocellulose, and PVDF membranes or PAGE gels[1] with the detection sensitivity similar to that of Coomassie stains. Most other stains produce high backgrounds on nylon membranes due to strong charge interactions with the membrane. The mixing of equal amounts of Solution A and B activates the stain, which produces lavender stained protein bands. The staining is easily reversible with an EDTA solution so that the blot can be reused for Western blotting or for amino acid sequence analysis (PVDF). R-PROB does not contain the toxic chemicals cacodylic acid or potassium ferrocyanide.
Autres remarques
Compatible with nylon, nitrocellulose, and PVDF membranes as well as polyacrylamide gels.
Composants de kit seuls
Réf. du produit
Description
- Solution A 500 mL
- Solution B 500 mL
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
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Sensitivity and mass accuracy for proteins analyzed directly from polyacrylamide gels: Implications for proteome mapping
Rachel R.
Electrophoresis (1997)
Articles
Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.
Protocoles
Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.
Contenu apparenté
Instructions
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When using Product RPROB, Reversible Protein Detection Kit, to stain protein on PAGE gels, can the protein be transferred from the gel to a membrane after destaining?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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