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SCM123

ChondroMAX Differentiation Medium

Synonyme(s) :

MSC chondrocyte media, chondrocyte differentiation media, chondrogenesis media

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100 ML

441,00 $

441,00 $


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A propos de cet article

NACRES:
NA.75
UNSPSC Code:
12352207

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dry ice

storage temp.

−20°C

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SCM121SCM045C-28012
storage temp.

−20°C

storage temp.

-

storage temp.

-

storage temp.

-

shipped in

dry ice

shipped in

dry ice

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General description

Mesenchymal stem cells (MSCs) are adult stem cells, which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (cartilage). Articular hyaline cartilage has poor regenerative capacity, and the loss of its function is, in the long term, often painful and debilitating. Therefore, attempts have been made to study chondrogenesis or to replace damaged cartilage tissue with MSC derived stem cells.
ChondroMAX Differentiation Medium is a ready-to-use xeno-free mesenchymal stem cell chondrogenesis differentiation media. ChrondroMAX produces cells with elevated levels of sulfated proteoglycans, analyzed by Alcian-Blue staining, after 3 weeks of MSC pellet differentiation compared to other chrondrogenesis differentiation media. ChondroMAX is an excellent research tool to study chondrocyte differentiation, chondrogenesis and wound healing.

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Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Classe de stockage

8B - Non-combustible corrosive hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

Protocoles

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

Questions

1–2 of 2 Questions  
  1. Hi, can it be used for O9-1 Mouse Cranial Neural Crest Cell Line differentiation? Thank you.

    1 answer
    1. ChondroMAX Differentiation Medium is optimized for the differentiation of human mesenchymal stem cells into chondrocytes. We have not tested it for O9-1 Mouse Cranial Neural Crest Cell Line differentiation.
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/882/447/scm123pisrev1124-ms.pdf

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  2. Hello Will this need to be incubated in a 5% CO2? What if kept in a atmospheric CO2 level? Will we need to add aby buffering agents to maintain good PH level? Any recommendations?

    1 answer
    1. This material should be incubated in 5% CO2. Loosen the cap of the tube to allow gas exchange and incubate upright at 37 °C and 5% CO2. You will not need to add any buffering agents to this product. Please view the following link for more information:

      https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/stem-cell-culture/mesenchymal-stem-cell-culture-protocols

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