The microprotein kit TP0200 is not intended for use above 1 mg/ml
TP0200
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification
sufficient for ~50 manual assays
Synonyme(s) :
Total Protein Assay Kit
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| Taille/Référence | Disponibilité | Prix |
|---|---|---|
1 kit | Disponible dès aujourd'huiDeMilliporeSigma Canada Ltd. | 190,00 $ |
A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352202
190,00 $
Disponible dès aujourd'huiDétails
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderdescription
wavelength 500-750nm
Quality Segment
usage
sufficient for ~50 manual assays
protein detection level measuring range
0-1 mg/mL
parameter
18-26 °C temperature, 200 μL sample volume
storage temp.
2-8°C
General description
The biuret and Lowry procedures are used for protein determination. The former is widely used for clinical assays. The latter, though more sensitive, is used for investigative work and is limited by poor stability of combined reagents, non-reproducibility of color, especially at low protein concentration, and nonlinear chromogenic response with protein concentration. Ohnishi and Barr modified the biuret reagent for the Lowry procedure, thereby simplifying it while improving the stability of the combined reagent.
Application
Total Protein Kit, Micro-Lowry, Onishi & Barr Modification has been used to determine total soluble protein concentrations:
- in the dialyzates/extracts of gliadins
- in the extracts of flour (Avena sativa and Triticum durum) and seeds (Chenopodium quinoa, Salvia hispanica L.)
- in the stored samples of rat liver microsomal fractions
Biochem/physiol Actions
Colorimetric, Endpoint.assay. Ohnishi and Barr′s modification of micro Lowry method. According to procedure, dilute biuret reagent reacts with peptide bonds to yield a purple-blue complex, the color of which is intensified by the addition of phenol reagent. Absorbance, read at 550-750 nm, is used to determine results from a standard curve.
1 of 1
Cet article | |||
|---|---|---|---|
| description sufficient for ~50 manual assays | description - | description - | description suitable for determination of total protein by Lowry method, 1.9-2.1 N |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| description wavelength 500-750nm | description - | description - | description - |
| parameter 200 μL sample volume, 18-26 °C temperature | parameter - | parameter - | parameter - |
| usage sufficient for ~50 manual assays | usage sufficient for ~200 assays | usage - | usage - |
| protein detection level measuring range 0-1 mg/mL | protein detection level measuring range - | protein detection level measuring range - | protein detection level measuring range - |
| storage temp. 2-8°C | storage temp. - | storage temp. - | storage temp. room temp |
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS & Tarif
Mot-clé
Danger
Codes de danger
Mentions de précaution
Hazard Classifications
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1
Classe de stockage
8A - Combustible corrosive hazardous materials
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
-
does it works for samples with 11 mg/ml?
1 answer-
Helpful?
-