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70954

Rosetta(DE3) Competent Cells - Novagen

Escherichia coli, rod shaped

Synonym(s):

BL21 derivatives

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0.4 ML

CHF 292.00

1 ML

CHF 499.00

CHF 292.00

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About This Item

UNSPSC Code:
41106202
NACRES:
NA.81

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Product Name

Rosetta(DE3) Competent Cells - Novagen, Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

biological source

Escherichia coli

Quality Level

100

manufacturer/tradename

Novagen®

storage condition

OK to freeze

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

shipped in

wet ice

storage temp.

−70°C

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This Item
709207095371058
Rosetta(DE3) Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

70954

Rosetta(DE3) Competent Cells - Novagen

Rosetta(DE3)pLacI Competent Cells - Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Sigma-Aldrich

70920

Rosetta(DE3)pLacI Competent Cells - Novagen

Rosetta Competent Cells - Novagen Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

70953

Rosetta Competent Cells - Novagen

RosettaBlue Competent Cells - Novagen RosettaBlue host strains are NovaBlue derivatives that combine high transformation efficiency and with enhanced expression of eukaryotic proteins that contain codons rarely used in E. coli.

Sigma-Aldrich

71058

RosettaBlue Competent Cells - Novagen

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

biological source

Escherichia coli

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

General description

Genotype: F-ompT hsdSB(rB- mB-) gal dcm (DE3) pRARE (CamR)

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges us to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. These strains supply tRNAs for AGG, AGA, AUA, CUA, CCC, GGA codons on a compatible chloramphenicol-resistant plasmid. Thus the Rosetta strains provide for “universal” translation which is otherwise limited by the codon usage of E. coli. The tRNA genes are driven by their native promoters. In Rosetta(DE3)pLysS, the rare tRNA genes are present on the same plasmids that carries the T7 lysozyme gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Other Notes

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta(DE3) Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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