D5197
SigMatrix Ultra Serum Diluent
Synonym(s):
Serum Diluent
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100 ML
CHF 839.00
500 ML
CHF 3’070.00
5 X 100 ML
CHF 3’070.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.25
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biological source
human
Quality Level
recombinant
expressed in rice
form
solution
concentration
18—22 mg/mL protein
technique(s)
LC/MS: suitable
shipped in
wet ice
storage temp.
−20°C
1 of 4
This Item | A4414 | D5322 | 936B |
|---|---|---|---|
| technique(s) LC/MS: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) immunohistochemistry: suitable |
| biological source human | biological source Porcine | biological source synthetic | biological source - |
| grade GMP | grade - | grade - | grade - |
| Quality Level 500 | Quality Level 200 | Quality Level 200 | Quality Level 500, 600 |
| form solution | form lyophilized powder | form - | form buffered aqueous solution (green) |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
General description
For more information on other Sigmatrix products visit the Sigmatrix Selection Guide.
Application
This solution is provided ready-to-use as a 2% (w/v) recombinant HSA (Human Serum Albumin) in PBS (Phosphate Buffered Saline), pH 7.4. This formulation has been shown to be appropriate for use as a sample diluent or matrix for preparing calibrants/controls for clinical diagnostic LC-MS/MS assays. Beneficially, as the HSA source is recombinant, the matrix should not contain any measurable contaminants typically found in even doubly stripped serum. SigMatrix Ultra has been successfully used in LC-MS/MS assays for clinically relevant concentrations of total testosterone (20–2,000 pg/mL), estradiol, and estrone (2–1,000 pg/mL). Calibrators prepared in SigMatrix Ultra showed good linearity (r2 >0.97) and no interference at the lowest calibrator concentrations for all analytes tested. Additionally, extracted blank SigMatrix Ultra spiked with analyte specific isotopicallylabelled internal standards showed no interferences above the lower limits of quantitation at the retention time of the respective analytes.
Physical form
SigMatrix Serum Diluent is an aqueous solution containing 2% highly purified recombinant human albumin (expressed in rice) in phosphate buffered saline (pH 7.4) with no preservatives
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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