The protocol is published in the product datasheet, available in the 'DOCUMENTATION' section of this page, under the 'Mor Documents' tab. Please see the link below to review:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf
MINI26
PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling
Distributed for Phanos Technologies
Synonyme(s) :
Red PKH membrane label kit
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1 KIT
365.00 CHF
365.00 CHF
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A propos de cet article
NACRES:
NA.32
UNSPSC Code:
12352207
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PKH26 Red Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling, Distributed for Phanos Technologies
packaging
pkg of 1 kit
manufacturer/tradename
Distributed for Phanos Technologies
storage condition
protect from light
technique(s)
flow cytometry: suitable
fluorescence
λex 551 nm; λem 567 nm (PKH26 dye)
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
ambient
storage temp.
room temp
Quality Level
1 of 4
Cet article | PKH26GL | MINI67 | PKH26PCL |
|---|---|---|---|
| technique(s) flow cytometry: suitable | technique(s) - | technique(s) flow cytometry: suitable | technique(s) - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - |
| manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename Distributed for Phanos Technologies | manufacturer/tradename - |
| storage condition protect from light | storage condition protect from light | storage condition protect from light | storage condition - |
| fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 551 nm; λem 567 nm (PKH26 dye) | fluorescence λex 490 nm; λem 502 nm (PKH67 dye) | fluorescence - |
| application(s) cell analysis | application(s) - | application(s) cell analysis | application(s) - |
Application
PKH26 red fluorescent cell linker mini kit has been used for:
General description
This kit provides enough dye for up to 25 samples of 20 million cells each and enough diluent for up to 5 samples of 20 million cells each, when using a 2 mL final staining volume containing a final concentration of 2 x 10-6 M of PKH26 dye. Users must determine the optimal dye and cell concentrations from their cell type(s) and experimental purposes.
Other Notes
For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.
Legal Information
CellVue is a registered trademark of Phanos Technologies
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Classe de stockage
3 - Flammable liquids
flash_point_f
57.2 °F - closed cup
flash_point_c
14 °C - closed cup
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Transplanted bone marrow stem cells relocate to infarct penumbra and co-express endogenous proliferative and immature neuronal markers in a mouse model of ischemic cerebral stroke
Zhang XM, et al.
BMC Neuroscience, 11(1), 138-138 (2010)
Maria Giovanna Gandolfi et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-03-04)
Exosomes derived from mesenchymal stem cells are extracellular vesicles released to facilitate cell communication and function. Recently, polylactic acid (PLA), calcium silicates (CaSi), and dicalcium phosphate dihydrate (DCPD) have been used to produce bioresorbable functional mineral-doped porous scaffolds-through thermally induced
Adipose tissue regeneration in vivo using micronized acellular allogenic dermis as an injectable scaffold
Lee HY, et al.
Aesthetic Plastic Surgery, 38(5), 1001-1010 (2014)
Human leukocyte antigen-G1 inhibits natural killer cytotoxicity through blocking the activating signal transduction pathway and formation of activating immunologic synapse
Yu Y, et al.
Human immunology, 69(1), 16-23 (2008)
Imaging of peripheral benzodiazepine receptor expression as biomarkers of detrimental versus beneficial glial responses in mouse models of Alzheimer's and other CNS pathologies
Ji B, et al.
The Journal of Neuroscience, 28(47), 12255-12267 (2008)
Articles
Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.
Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.
PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.
PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.
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MINI26-1KTの実験プロトコルを入手したいのですが、どこにありますか？
1 answer-
Helpful?
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Is it possible to lable cells with PKH26 at 4oC?
1 answer-
The membrane dyes will remain stable during the monoclonal staining at 4 deg C; however, capping of the monoclonal antibodies is highly probable if the general cell membrane labeling is carried out at ambient temperature subsequent to antibody labeling. As additional information may be required to address the question completely, please navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquiries" under the Products Section with all the information needed so that a member of the Technical Service team can reach out to assist further.
For more information, please refer to the product data-sheet below: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf.
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