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Scalable Tangential Flow Filtration (TFF) for Downstream Processing of Viral Vectors for Gene Therapy and Vaccine Production

Our overview of scalable tangential flow filtration for viral vector production will provide key considerations for developing and optimizing your downstream process.

Water Determination in Liquefied Petroleum Gas using GC BID and Ionic Liquid Column Watercol™

Using Watercol™ GC columns in conjunction with GC-BID can provide a reliable and practical option for Water determination in petrochemical feedstocks.

Toolkit für erneuerbare Elektrizität

Das Toolkit für Anbieter von erneuerbarem Strom hilft Anbietern, die Grundlagen der erneuerbaren Energien zu erlernen und Projekte in die Tat umzusetzen.

Toolkit per l'elettricità rinnovabile

Il Toolkit per i fornitori di energia elettrica rinnovabile aiuta i fornitori a conoscere le basi dell'energia elettrica rinnovabile e a realizzare i progetti.

Renewable Electricity Toolkit

The Renewable Electricity Supplier Toolkit helps suppliers learn renewable electricity basics and how to bring projects to life.

Rat Dermal Fibroblasts (RDF) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

StableCell™ Trypsin Solutions

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

Subculture of Adherent Cell Lines

Cell culture protocol for passaging and splitting adherent cell lines using trypsin EDTA. Free ECACC handbook download.

Testing Cells for Bacterial and Fungal Contamination

Cell culture protocol for testing cell lines for bacterial and fungal contamination. Free ECACC handbook download.

Thawing of Frozen Cell Lines

Cell culture protocol for proper thawing of cryopreserved cell lines.

Trypsin Inhibitors

Natural trypsin inhibitors (serpins) regulate protein activation and catabolism by inhibiting serine proteases in vivo.

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Initiating a Starter Culture

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Antibiotics in Plant Tissue Culture Protocol

Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.

Explant Sterilization – Plant Tissue Culture Protocol

Surface sterilizing plant material is an important for plant tissue culture. Commonly used disinfectants for plant tissue culture and methods for disinfecting seeds are included.

Gelling Agents – Plant Tissue Culture Protocol

Instructions for preparing alginate, agargel, and phytagel agar to solidify plant tissue culture media, with criteria for product selection.

Media Preparation – Plant Tissue Culture Protocol

Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use

Pathology Media – Plant Tissue Culture Protocol

Fungal and Bacterial Detection Media, Media Preparation and Storage, Media Composition Table.

Phycology Media in Plant Tissue Culture

Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.

Silver Thiosulfate – Plant Tissue Culture Protocol

Preparation instructions for 0.1 M Sodium Thiosulfate (STS) and 0.1 M Silver Nitrate stock solutions for laboratory use.

Vitamin Mixtures – Plant Tissue Culture Protocol

Preparation of Solutions from Packaged Powder, Media Preparation using Vitamin Solutions, Vitamin Mixtures Composition Table

ADCC Protocol using BioIVT Cryopreserved NK Effector Cells

Step-by-step protocol for ADCC assays using cryopreserved NK effector cells including tips and tricks, assay development, and data analysis methods.

Bovine Aortic Endothelial Cells (BAOEC) Culture Protocol

Technical information for working with bovine aortic endothelial cells including thawing, subculturing and cryopreservation.

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

In Vitro Expansion of CD34+/CD38- Human Hematopoietic Progenitor Cells in a Serum-free and Xeno-free Stem Cell Culture Media

In vitro culture of human hematopoietic stem cells in PromoCell’s serum-free/xeno-free HPC Expansion Medium DXF. Explore over 350 PromoCell products

Human Mesenchymal Stem Cells & Growth Media

Mesenchymal Stem Cells and Media enable in vitro expansion and differentiation, vital for various tissue sources.

Human Aortic Endothelial Cells (HAOEC) Culture Protocol

Technical information for working with human aortic endothelial cells including thawing, subculturing and cryopreservation.

Human Astrocytes (HA) Culture Protocol

Store the cryovials in a liquid nitrogen storage tank immediately upon arrival.

Human Bronchial Epithelial Cell line

Technical information for working with human bronchial epithelial cell line including thawing, subculturing and cryopreservation.

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