MBD6000
5R-Plex kit
Ultra-sensitive 16S NGS assay for degraded and low biomass DNA
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A propos de cet article
Code UNSPSC :
41106302
Nomenclature NACRES :
NA.84
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Utilisation
Preparing 96 samples for sequencing
Technique(s)
DNA amplification: suitable
DNA sequencing: suitable
Conditions d'expédition
dry ice
Température de stockage
-10 to -25°C
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Cet article | SEQXE | MBD0027 | AMP3100 |
|---|---|---|---|
| technique(s) DNA amplification: suitable, DNA sequencing: suitable | technique(s) whole genome amplification: suitable | technique(s) DNA extraction: suitable, DNA sequencing: suitable, PCR: suitable | technique(s) DNA amplification: suitable |
| usage Preparing 96 samples for sequencing | usage - | usage - | usage - |
| shipped in dry ice | shipped in wet ice | shipped in dry ice | shipped in - |
| storage temp. -10 to -25°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Description générale
The 16S ribosomal RNA gene (16S rRNA) is a common bacterial marker, used as a target for microbial community profiling in microbiome metagenomic studies. The gene is composed of nine variable regions (V1-V9) interspersed between conserved regions. The most common 16S rRNA NGS assays target one or two regions using a single set of primers (e.g., V3-V4). However, this approach usually results in a poor bacterial detection and classification rate when the DNA input is of low-quality or when applying it on extremely low biomass samples. The 5R-PLEX kit offers a complete solution to challenging biological samples where the standard 16S sequencing protocols fail to perform. Sample types include:
- Formalin-Fixed, Paraffin-Embedded (FFPE) tissue[1]
- Cancerous tumor tissue
- Degraded or damaged DNA
- Low biomass samples
- Fossil-derived and ancient DNA
Caractéristiques et avantages
- The 5R-PLEX Kit provides high profile sequencing from degraded, damaged or low biomass samples
- Multiplexed NGS assay targets 5 variable regions of the 16S rRNA gene in a single primer pool
- Includes positive control for trouble shooting experiments.
- The kit can detect as low as 1pg of highly degraded bacterial DNA with an average fragment size of 260bp.
- Kit includes low bioburden reagents
- Includes exclusive access to M-CAMP™ platform featuring an innovative algorithm designed to reconstruct a single coherent microbial profiling and perform comprehensive analysis.
Notes préparatoires
The shelf life of all reagents provided in the kit is 12 months when stored properly. Store all components at -20 °C.
Autres remarques
- 5R-PLEX PCR1 Primer mix- 25 μL
- 5R-PLEX PCR2 Forward Primer mix- 25 μL
- 5R-PLEX index plate 96-plate
- Water, microbial DNA-free 10X- 1.5 mL
- HF DNA Polymerase- 0.1 mL
- 5X HF buffer 2 mL
- dNTP′s- 0.2 mL
- Elution Buffer (EB), microbial DNA-free- 8 mL
- 5R-PLEX Positive Control (10 ng/μL)- 30 μL
Kit component details can be found in the User guide.
Informations légales
M-Camp is a trademark of Merck KGaA, Darmstadt, Germany
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3
Code de la classe de stockage
10 - Combustible liquids
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