[Přejít k obsahu](https://www.sigmaaldrich.com#main-content) [](https://www.sigmaaldrich.com/CZ/cs) Produkty Nákupní košík0 CZCS Produkty ProduktyAplikaceSlužbyZdrojePodpora [Přihlášení / Registrovat se](https://www.sigmaaldrich.com/oidc-sign-in) [Dohledání objednávky](https://www.sigmaaldrich.com/CZ/cs/order-lookup) [Rychlá objednávka](https://www.sigmaaldrich.com/CZ/cs/quick-order) Nákupní košík0 [Home](https://www.sigmaaldrich.com/CZ/en)[Applications](https://www.sigmaaldrich.com/CZ/en/applications)[Genomics](https://www.sigmaaldrich.com/CZ/en/applications/genomics)Next-Generation Sequencing # Next-Generation Sequencing  Next-generation sequencing (NGS) is a term that broadly captures several related technologies that enable massively parallel or deep sequencing coverage for a selected region or the entire genome of an organism. Essential within the discipline of genomics-based research, sequencing technologies have existed for decades. However, the continual advancement of NGS or massively parallel DNA and RNA sequencing technologies have provided researchers with increases in genome-wide sequencing coverage and data analysis tools while rapidly decreasing in cost. Applications for NGS extend beyond whole genome analysis as it has significant implications for recent advancements in fundamental genomics and disease research alike. * * * ## Related Products Slide 1 of 5 1 of 1 [ \ Sigma-Aldrich \ SEQXE \ SeqPlex DNA Amplification Kit](https://www.sigmaaldrich.com/CZ/cs/product/sigma/seqxe) Rychlé zobrazení [ \ Sigma-Aldrich \ W4502 \ Water](https://www.sigmaaldrich.com/CZ/cs/product/sigma/w4502) Rychlé zobrazení [ \ Sigma-Aldrich \ S9430 \ SYBR™ Green I nucleic acid gel stain](https://www.sigmaaldrich.com/CZ/cs/product/sial/s9430) Rychlé zobrazení [ \ Sigma-Aldrich \ NA1020 \ GenElute™ PCR Clean-Up Kit](https://www.sigmaaldrich.com/CZ/cs/product/sigma/na1020) Rychlé zobrazení [ \ Sigma-Aldrich \ WTA2 \ Complete Whole Transcriptome Amplification Kit](https://www.sigmaaldrich.com/CZ/cs/product/sigma/wta2) Rychlé zobrazení * * * ## Featured Categories [](https://www.sigmaaldrich.com/CZ/en/products/chemistry-and-biochemicals/biochemicals/agarose) [Agarose: Properties and Research Applications](https://www.sigmaaldrich.com/CZ/en/products/chemistry-and-biochemicals/biochemicals/agarose) Agarose bioreagents cater to cell culture and molecular biology applications, offering ready-to-pour agarose gels for RNA/DNA electrophoresis. [Shop Products](https://www.sigmaaldrich.com/CZ/en/products/chemistry-and-biochemicals/biochemicals/agarose) [](https://www.sigmaaldrich.com/CZ/en/products/molecular-biology-and-functional-genomics/microbiome) [Microbiome](https://www.sigmaaldrich.com/CZ/en/products/molecular-biology-and-functional-genomics/microbiome) Comprehensive Gut Microbiome Analysis: Uncover a holistic solution encompassing sample prep, sequencing, bioinformatics, and statistics. From 16S to WGS. [Shop Products](https://www.sigmaaldrich.com/CZ/en/products/molecular-biology-and-functional-genomics/microbiome) * * * Overview Related Articles & Protocols Support ## NGS Methods Overview While the methodology and reagents for NGS are continuously evolving, there are now numerous NGS systems that are available to researchers. Commonly used platforms incorporate the use of several critical steps in the NGS workflow, including sample or library preparation, cluster generation, sequencing, and data analysis. Sample preparation typically involves either DNA amplification or the addition of sequence linkers or adaptors. Cluster generation of each DNA sequence is when DNA containing the covalently attached linker hybridizes to a solid surface for bridge PCR amplification, or by alternate methods such as emulsion PCR. Additionally, there are many DNA sequencing methods, including sequencing by ligation, sequencing by synthesis, pyrosequencing, and ion semiconductor sequencing. Each sequencing method involves varying reaction steps and chemistries that ultimately determine the length of each sequence (read length), error rate, and reagent cost. ## [](https://www.sigmaaldrich.com)Analytical Approaches for NGS Data Analyses A final element for all NGS workflows is the critical data analysis step that occurs after sequencing. While each NGS platform and workflow produce an enormous amount of digital information captured on computers, the raw data set must be analyzed by bioinformaticians using a continuously increasing number of analytical tools for read alignment and mapping, such as Bowtie, Galaxy, and many others. Many of the developments in the field of NGS technologies has come from the merger of numerous scientific fields to develop and optimize the analysis and interpretation of such large data sets. Depending on the specific application needs, researchers are now able to use these powerful tools to sequence entire genomes, exomes, or transcriptomes for fundamental and disease research studies. [](https://www.sigmaaldrich.com/documents-search) [Looking for More Specific Information?](https://www.sigmaaldrich.com/documents-search) Visit our document search for data sheets, certificates and technical documentation. [Find Documents](https://www.sigmaaldrich.com/documents-search) ## Related Articles - [Custom Next-Gen Sequencing Oligos](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/custom-next-gen-sequencing-oligos) Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments. - [siRNA Universal Neg. Controls](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/mission-sirna-universal-negative-controls) MISSION siRNA Negative Controls ensure mRNA knockdown efficiency baseline; tested in human, rat, and mouse cells, designed without gene homology. - [Positive Control siRNA](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/mission-positive) MISSION Positive Control siRNA, validated for siRNA silencing experiments, saves time in gene silencing research. - [Water for Next-Generation Sequencing](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/water-for-next-generation-sequencing) Improve NGS performance and reliability by optimizing water quality at every step of the workflow, from fragmentation to cleaning to sequencing. - [See All (18)](https://www.sigmaaldrich.com/CZ/en/search/facet-search?focus=sitecontent&term=facet-search) ## Related Protocols - [SeqPlex DNA Amplification Kit Protocol](https://www.sigmaaldrich.com/CZ/en/technical-documents/protocol/genomics/next-gen-sequencing/seqxe-protocol) SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification. - [Annealing Oligonucleotides Protocol](https://www.sigmaaldrich.com/CZ/en/technical-documents/protocol/genomics/pcr/annealing-oligos) Annealing is the process of heating and cooling two single-stranded oligonucleotides with complementary sequences. - [SEQR - SeqPlex RNA Amplification Kit Protocol](https://www.sigmaaldrich.com/CZ/en/technical-documents/protocol/genomics/pcr/seqr) The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms. - [GenomePlex® Single Cell Whole Genome Amplification Kit (WGA4) - Protocol](https://www.sigmaaldrich.com/CZ/en/technical-documents/protocol/genomics/pcr/single-cell-whole-genome-amplification-kit) GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA - [Complete Whole Transcriptome Amplification Kit Protocol (WTA2)](https://www.sigmaaldrich.com/CZ/en/technical-documents/protocol/genomics/pcr/whole-transcriptome-amplification-kit) WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias - [See All (5)](https://www.sigmaaldrich.com/CZ/en/search/facet-search?focus=sitecontent&term=facet-search) ### Find More Articles and Protocols Enter Keywords Search ## How Can We Help In case of any questions, please submit a [customer support request](https://www.sigmaaldrich.com/CZ/en/support/customer-support) or talk to our customer service team: Email [custserv@sial.com](mailto:custserv@sial.com) or call +1 (800) 244-1173 ## Additional Support - [Chromatogram Search](https://www.sigmaaldrich.com/chromatogram-search) Use the Chromatogram Search to identify unknown compounds in your sample. - [Calculators & Apps](https://www.sigmaaldrich.com/CZ/en/support/calculators-and-apps) Web Toolbox - science research tools and resources for analytical chemistry, life science, chemical synthesis and materials science. - [Customer Support Request](https://www.sigmaaldrich.com/CZ/en/support/customer-support) Customer support including help with orders, products, accounts, and website technical issues. - [FAQ](https://maestro.my.site.com/knowledgeportal/s/) Explore our Frequently Asked Questions for answers to commonly asked questions about our products and services. * * * __Featured Articles__ [Custom Next-Gen Sequencing Oligos](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/custom-next-gen-sequencing-oligos) Regardless of next-generation sequencing (NGS) platform, universal and index adapter sequences are required for the proper assembly of sample fragments. [siRNA Universal Neg. Controls](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/mission-sirna-universal-negative-controls) MISSION siRNA Negative Controls ensure mRNA knockdown efficiency baseline; tested in human, rat, and mouse cells, designed without gene homology. [Positive Control siRNA](https://www.sigmaaldrich.com/CZ/en/technical-documents/technical-article/genomics/next-gen-sequencing/mission-positive) MISSION Positive Control siRNA, validated for siRNA silencing experiments, saves time in gene silencing research. Nejvyšší __Chcete-li pokračovat, musíte se přihlásit.__ Abyste mohli pokračovat ve čtení, přihlaste se nebo vytvořte účet. 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