341635

Human Fibronectin

from human plasma, liquid, suitable for cell culture

• Synonyma: Fibronectin, Human Plasma, Fibronectin purified from human plasma, Human Fibronectin
• Číslo CAS: 86088-83-7
• MDL number: MFCD00078962
• UNSPSC Code: 12352202
• NACRES: NA.75
• Form: solution
• Technique(s): cell culture | mammalian: suitable

Vlastnosti

• Název produktu: Fibronectin, Human Plasma, solution, suitable for cell culture
• description: Merck USA index - 14, 4075
• Quality Segment: 200
• form: solution
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• shipped in: wet ice
• storage temp.: −20°C

Popis

General description

Fibronectin is a glycoprotein belonging to the extracellular matrix (ECM) protein family. Fibronectin contains two polypeptides (α and β chains) linked by two disulfide bonds near the carboxyl termini to form a dimer.Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.

Application

The human plasma fibronectin has been used for coating surfaces such as poly-l-lysine (PLL)-coated nanochips, polyacrylamide (PAAm) hydrogel substrates, glass slides and more, providing a substrate for the adhesion and culture of cells in various experimental setups.

Biochem/physiol Actions

Human fibronectin protein plays crucial roles in various cellular behaviors such as cell attachment, migration, embryogenesis, wound healing, tissue integrity, and malignant processes. One of the key features enabling fibronectin to support cell adhesion is the presence of the Arg-Gly-Asp (RGD) tripeptide. It has been extensively studied in areas like fibronectin coating on polystyrene (PS) and poly(lactic-co-glycolic acid) (PLGA) surfaces. Additionally, fibronectin has been the subject of significant research regarding its involvement in regulating processes such as differentiation-to-growth switching, tubulin monomer growth, cell cycle status, cell spreading, and motility.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, pH 7.3.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Contaminants: none detected by IEP with antiserum to fibronectin or normal human serum

single major band by SDS-PAGE

Other Notes

Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol.59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.


Brown, S., et al. 2002. Nature418, 200.


Kim, M., FEBS Letters581 1917.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Toxicity: Standard Handling (A)

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• Co se děje?: WGK 2