539131

Protease Inhibitor Cocktail I

lyophilized, for the inhibition of aspartic, cysteine, serine, metalloproteases and aminopeptidases

• Synonyma: Protease Inhibitor Mix, Protease inhibitor
• UNSPSC Code: 12352200
• NACRES: NA.28
• Form: lyophilized
• Solubility: water: soluble
• Storage temp.: −20°C

Vlastnosti

• Název produktu: Protease Inhibitor Cocktail Set I, A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.
• form: lyophilized
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated (hygroscopic)
• input: general use sample(s)
• solubility: water: soluble
• shipped in: wet ice
• storage temp.: −20°C

Popis

General description

Protease Inhibitor Cocktail Set I A is specially formulated containing five protease inhibitors that will inhibit a broad range of proteases and esterases.

Application

Protease Inhibitor Cocktail Set I have been used as a supplement in:

• cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
• RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
• sucrose solution to homogenize mice cortices for myelin isolation

Protease Inhibitor Cocktail Set I – Calbiochem has been used in lysis buffer to lyse cells and perform western blotting.

Biochem/physiol Actions

Inhibits a broad range of proteases and esterases.

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

Features and Benefits

• Protease Inhibitor Cocktails offer protection of target protein against degradation in crude extracts
• Ready for reconstitution

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

Reconstitute each vial with 1 ml dH2O to yield a 100X stock solution.

Other Notes

The presence of EDTA may interfere with the purification of His•Tag fusion proteins if a cell extract is applied directly to IMAC resins.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

Bezpečnostní informace

• Piktogramy: GHS08,GHS05,GHS07
• Výstražné slovo: Danger
• Kódy nebezpečí: H314,H332,H373
• Bezpečnostní pokyny: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Skin Corr. 1A - STOT RE 2 Inhalation
• Cílové orgány: Respiratory Tract
• Skladovací třída: 8B - Non-combustible corrosive hazardous materials
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable