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69670-M

His•Bind® Resin

Synonyma:

uncharged IDA agarose beads

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O této položce

NACRES:
NA.56
UNSPSC Code:
41106500
Technický servis
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form

liquid

manufacturer/tradename

Novagen®

storage condition

do not freeze

application(s)

life science and biopharma

storage temp.

2-8°C

General description

His•Bind Resin is an uncharged IDA agarose resin. The binding capacity is approximately 8 mg/ml bed volume. His•Bind Resin is well suited to small to medium scale purifications using either gravity flow columns or batch methods.

Application

HIS-BIND® resin have been used: in purification of HIS TAG® fusion proteins by metal chelation chromatography, for purification of the bacterially produced OCRL1WT/VAR phosphatase domain to study heterogeneity in Lowe Syndrome, in protein purification to study anaerobic phloroglucinol degradation by Clostridium scatologenes, for isolation of His6-tagged proteins for studying the role of human repressor activator protein 1 (RAP1) and glial fibrillary acidic protein (GFAPɛ)proteins in increasing γ-secretase activity in a yeast model system.

Biochem/physiol Actions

HIS-BIND® resin helps in the purification of proteins containing the His-tagged sequence by the process of metal chelation chromatography. It utilizes the charge buffer provided in the HIS-BIND® Buffer Kit, that binds the His-tagged sequence effectively with Ni2+ or Co2+ cations that are immobilized onto the HIS-BIND® resin. The target protein can be easily recovered by eluting with imidazole. The HIS-BIND® resin is designed to be regenerated and reused multiple times, making it a cost-effective solution. Its versatility allows for protein purification under gentle, non-denaturing conditions, or in the presence of either guanidine or urea, depending on the specific requirements of the purification process.

Features and Benefits

  • Reusable many times
  • Compatible with HIS-BIND® Buffer Kit
  • Compatible with 1 mM THP

Legal Information

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Piktogramy

Flame
GHS02

Výstražné slovo

Warning

Kódy nebezpečí

H226

Hazard Classifications

Flam. Liq. 3

Skladovací třída

3 - Flammable liquids

Bod vzplanutí (°F)

86.0 °F

Bod vzplanutí (°C)

30 °C

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Osvědčení o analýze (COA)

Lot/Batch Number

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