70794

BugBuster^® GST-Bind Purification Kit

Affinity purification of GST fusion proteins

• NACRES: NA.32
• UNSPSC Code: 41106500

Vlastnosti

• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze

Popis

General description

Affinity purification of GST fusion proteins

The BugBuster^® GST•Bind<TMSYMBOL></TMSYMBOL> Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag<TMSYMBOL></TMSYMBOL> fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.

Other Notes

•2 x 100 mlBugBuster Protein Extraction Reagent

•10,000 UBenzonase Nuclease, purity >90%

•10 mlGST•Bind Resin

•pkg/4Chromatography Columns

•2 x 100 ml10X GST Bind/Wash Buffer

•40 ml10X Glutathione Reconstitution Buffer

•1 gGlutathione, Reduced

Legal Information

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Bezpečnostní informace

• Piktogramy: GHS02
• Výstražné slovo: Warning
• Kódy nebezpečí: H226
• Bezpečnostní pokyny: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Skladovací třída: 3 - Flammable liquids
• Bod vzplanutí (°F): 96.8 °F
• Bod vzplanutí (°C): 36 °C
• WGK: WGK 3