IP05
Protein G Plus/Protein A Agarose Suspension
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Synonyma:
Affinity resin, Protein G/Agarose
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O této položce
NACRES:
NA.56
UNSPSC Code:
41116133
Technický servis
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slurry (Liquid)
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
technique(s)
protein purification: suitable
suitability
suitable for microbiology
shipped in
wet ice
storage temp.
2-8°C
Související kategorie
General description
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Application
Immunoprecipitation (see comments)
Physical form
33% slurry in PBS.
Other Notes
Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Skladovací třída
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
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Související obsah
We manufacture IP and antibody purification agarose beads that purify immunoglobulins and IgG fractions. Our kits also provide a total antibody purification process
Globální číslo obchodní položky
| Skladová položka | GTIN |
|---|---|
| IP05-1.5ML | 04055977220223 |
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
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