11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Synonyma: buffer
• UNSPSC Code: 41105600
• NACRES: NA.54

Vlastnosti

• form: solution
• packaging: pkg of 3 × 1 mL
• manufacturer/tradename: Roche
• technique(s): PCR: suitable, whole genome amplification: suitable
• storage temp.: −20°C

Popis

General description

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Application

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).

Physical form

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Other Notes

For general laboratory use.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• WGK: WGK 1
• Bod vzplanutí (°F): does not flash
• Bod vzplanutí (°C): does not flash