This product is a Saline-Tris-EDTA (STE) buffer that is ready-to-use. It has a pH specification of 7.6 to 8.0, with a lot-specific pH provided on the certificate of analysis (CofA). This buffer is prepared using Tris base, which has a pH of about 11 in water; the pH is then lowered to the 7.6 to 8.0 range by using hydrochloric acid. Since the pKa of Tris is 8.1, one could say that the buffer is 10 mM in Tris, and just a little over 5 mM in HCl.
85810
STE buffer solution
BioUltra, Molecular Biology, pH 7.8
Synonyma:
TRIS NaCl EDTA buffer solution
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Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
100 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 350,00 Kč |
500 mL | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 6 190,00 Kč |
O této položce
NACRES:
NA.25
UNSPSC Code:
41105319
2 350,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Molecular Biology
Quality Segment
product line
BioUltra
form
liquid
impurities
DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
pH
7.8±0.2 (25 °C)
cation traces
Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg
λ
neat
UV absorption
λ: 260 nm Amax: 0.03, λ: 280 nm Amax: 0.01
Application
- Safer DNA extraction from plant tissues using sucrose buffer and glass fiber filter.: The study demonstrates a novel method for DNA extraction from plant tissues using STE buffer solution, ensuring higher safety and efficiency. This approach improves DNA yield and quality, essential for plant genetic research (Takakura et al., 2012).
- In vitro DNA fragmentation of mitochondrial DNA caused by single-stranded breakage related to macroplasmodial senescence of the true slime mold, Physarum polycephalum.: This study employs STE buffer solution to investigate mitochondrial DNA fragmentation in the slime mold Physarum polycephalum, providing valuable insights into cellular aging processes (Abe et al., 2000).
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Tato položka | |||
|---|---|---|---|
| description BioUltra, Molecular Biology, pH 7.8 | description BioUltra, >0.044 M Na2HPO4, >0.022 M NaH2PO4 | description - | description BioUltra, >0.1 M Na2CO3, >0.2 M NaHCO3 |
| grade Molecular Biology | grade - | grade - | grade - |
| product line BioUltra | product line BioUltra | product line - | product line BioUltra |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 100 |
| form liquid | form liquid | form liquid | form liquid |
| pH 7.8±0.2 (25 °C) | pH 7.2±0.2 | pH - | pH 9.5±0.2 |
| cation traces Co: ≤1 mg/kg, Li: ≤1 mg/kg, Cr: ≤1 mg/kg, Ca: ≤5 mg/kg, Ni: ≤1 mg/kg, Fe: ≤1 mg/kg, Bi: ≤1 mg/kg, Al: ≤1 mg/kg, Mn: ≤1 mg/kg, Sr: ≤1 mg/kg, Pb: ≤1 mg/kg, Ba: ≤1 mg/kg, K: ≤20 mg/kg, Mg: ≤1 mg/kg, Cu: ≤1 mg/kg, Zn: ≤1 mg/kg, Mo: ≤1 mg/kg, Cd: ≤1 mg/kg | cation traces - | cation traces - | cation traces - |
Skladovací třída
12 - Non Combustible Liquids
Co se děje?
nwg
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
Eyeshields, Gloves
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Why do we prepare the buffer using Tris‑HCl and then adjust the pH to 8, instead of starting directly with Tris base since it is already closer to pH 8?
1 answer-
Helpful?
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Hello, is your item STE Buffer Molecular grade #85810 composed of these reagents: 0.1M NaCl, 1mM EDTA, 50mM Tris-HCl ? Is the pH stable overtime? Thank you for your reply.
1 answer-
This product is a Molecular Biology grade. The solution contains NaCl at 100 mM, Tris HCl at 10 mM, and Na2EDTA at 1 mM. The pH range is 7.6 - 8.0. The product has been filtered using a 0.22 um pore size filter membrane and is tested for RNase, DNase, Protease and Phosphatase activity.
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