The decline in activity over time due to storage conditions has not been determined. Other Proteinase K products may ship at ambient temperature, indicating little or no degradation of the material is expected. However, this items ships on ice. Maintaining the shipping and storage temperature at -20°C is recommended for this product in order to assure optimum performance.
P2308
Proteinase K from Tritirachium album
lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein
Synonyma:
Endopeptidase K
Přihlásit pro zobrazení organizačních a smluvních cen.
Vybrat velikost
Změnit zobrazení
| Vám/SKU | Dostupnost | Cena |
|---|---|---|
10 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 1 880,00 Kč |
5 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 220,00 Kč |
25 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 2 320,00 Kč |
100 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 10 100,00 Kč |
500 mg | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 33 000,00 Kč |
1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 39 200,00 Kč |
5 x 1 g | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 143 800,00 Kč |
O této položce
Číslo CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
254-457-8
NACRES:
NA.54
MDL number:
Číslo EC:
Specific activity:
≥30 units/mg protein
1 880,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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Molecular Biology
Quality Segment
product line
BioUltra
form
lyophilized powder
specific activity
≥30 units/mg protein
mol wt
28.93 kDa
composition
Protein, ≥90% biuret
impurities
≤1 ppm DNA
foreign activity
DNAse, Nickase and RNAse, none detected
shipped in
wet ice
storage temp.
−20°C
Application
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Other Notes
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
1 of 1
Tato položka | |||
|---|---|---|---|
| description lyophilized powder, Molecular Biology, BioUltra, ≥30 units/mg protein | description ≥30 units/mg protein | description buffered aqueous glycerol solution, Molecular Biology, ≥800 units/mL | description Reagents designed and manufactured under current ISO 13485 certification. |
| specific activity ≥30 units/mg protein | specific activity ≥30 units/mg protein | specific activity - | specific activity - |
| grade Molecular Biology | grade - | grade Molecular Biology | grade Molecular Biology |
| form lyophilized powder | form - | form buffered aqueous glycerol solution | form buffered aqueous glycerol solution |
| shipped in wet ice | shipped in - | shipped in - | shipped in wet ice |
| mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa | mol wt 28.93 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
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Výstražné slovo
Danger
Kódy nebezpečí
Bezpečnostní pokyny
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Cílové orgány
Respiratory system
Skladovací třída
11 - Combustible Solids
Bod vzplanutí (°F)
Not applicable
Bod vzplanutí (°C)
Not applicable
PPE (osobní ochranné prostředky)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Hi, I'd like to know what happens if the lyophilized proteinase K (P238) is not stored at -20C for at least a week.
1 answer-
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Is P2308 designed for antigen unmasking during IHC, and is there literature supporting this application?
1 answer-
Proteinase K has not been tested for use with IHC. Product P2308 is specifically tested for use in molecular biology, particularly for preparing cell lysates and releasing nucleic acids, as well as for PCR applications. While the enzyme has been used to digest hair (keratin) and may have other applications, there are no guarantees that P2308 would be suitable for antigen retrieval. No literature references stating that Proteinase K is commonly used for IHC were found.
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Can P2308 be dissolved in water for nucleic acid preparation? If so, at what concentration? Thanks!
1 answer-
This product may be dissolved in water for nucleic acid preparations at 1 mg/mL.
Please see the link below to review the product datasheet which includes preparation instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/116/320/p2308dat.pdfHelpful?
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How to prepare a solution of 20 mg/ml of pK (P2308-5MG)? In the section "Preparation Instructions" it mentions that I need to solve 1 mg in 1 ml of water. Does it means I'll have a final concentration of only 1 mg/ml?
1 answer-
The stock may also be prepared at 20 mg/ml concentration in 50 mM Tris-HCl, pH 8.0, containing 1 mM calcium chloride. This solution is stable for at least 12 months at 4°C. For additional information, please refer to the below publications where the product P2308 has been used.
1) https://www.nature.com/articles/s41598-017-17102-w#Sec9
2) https://www.frontiersin.org/articles/10.3389/fmicb.2018.00830/full#h3
3) https://elifesciences.org/articles/36187#s4Helpful?
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How to prepare Proteinase K solution to use in antigen retrieval of formalin fixed paraffin embedded tissue sections for the purpose of paraffin immunoflorescence? Which is the diluent of choice to prepare the solution from powder form of proteinase K.
1 answer-
This product is not tested for use in antigen retrieval. The product 21627, IHC Select Proteinase K protocol may be applied to this product, P2308. To prepare a stock solution, dissolve Proteinase K, P2308, at 0.2mg/mL in 0.05M Tris solution, pH 7.5.
For antigen retrieval in FFPA tissue sections (deparaffinized) the recommended dilution of the stock solution is 1:30.
End users would need to optimize final dilutionHelpful?
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What amount of Product P2308, Proteinase K, should I use for nucleic acid preparations?
1 answer-
Amounts for typical usage is 50-200 μg/mL.
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How should I store solutions of Product P2308, Proteinase K?
1 answer-
Stock solutions in 40% glycerol of at least 20 mg/ml can be stored in 10 mM Tris-HCl buffer, pH 7.5.with 1 mM calcium acetate (see product P4850). Solutions are also stable at pH 8 at 4°C.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the recommended pH range for Product P2308, Proteinase K?
1 answer-
Proteinase K is normally used in the pH range of 7.5-8.0.
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How should I prepare solutions of Product P2308, Proteinase K?
1 answer-
Solutions can be prepared in 25-50 mM Tris-HCl buffer, pH 8.0, containing 1-5 mM Calcium chloride.
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