46950

Fluorescein 5(6)-isothiocyanate

BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

• Synonyma: FITC, Fluorescein isothiocyanate
• Empirický vzorec (Hillův zápis): C₂₁H₁₁NO₅S
• Číslo CAS: 27072-45-3
• Molekulová hmotnost: 389.38
• UNSPSC Code: 12171500
• NACRES: NA.32
• PubChem Substance ID: 329757273
• EC Number: 248-207-7
• Beilstein/REAXYS Number: 1407295
• MDL number: MFCD00005063

Vlastnosti

• Quality Segment: 100
• product line: BioReagent
• assay: ≥90% (HPLC)
• form: powder
• mol wt: 389.38 g/mol
• color: orange to dark orange, yellow to dark yellow
• solubility: acetone: 0.1 g/10 mL, clear to very slightly hazy
• fluorescence: λ_ex 492 nm; λ_em 518 nm in 0.1 M phosphate pH 8.0
• suitability: suitable for fluorescence
• storage temp.: −20°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N=C=S)c1
• InChI: 1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChI key: MHMNJMPURVTYEJ-UHFFFAOYSA-N

Popis

General description

Fluorescein 5(6)-isothiocyanate, also known as FITC, is a weakly acidic hydroxyxanthene whose overall size is small, with both carboxylic acid and isothiocyanate substituents on the pendant phenyl ring. The dye has a moderately sized, nonplanar aromatic system. The isothiocyanate group reacts with nucleophilic substituents (e.g. amino, hydroxyl, thiol) of biomolecules to be attached as a fluorescent label. The maximum emission of the dye is at 519nm in the same solvent.

Application

Fluorescein 5(6)-isothiocyanate (FITC) labelled molecules are used in non-microscopic immunoassays and physiological tracing experiments. FITC is widely used in flow cytometry experiments for staining proteins, nuclei, and subnuclear particles. White blood cells (WBCs) are marked with FITC to study their response toward a chemoattractant, eventually being measured.

For fluorescent labeling of proteins (e.g. immunofluorescence); H Protein labeling for gel chromatography

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• PPE (osobní ochranné prostředky): dust mask type N95 (US), Eyeshields, Faceshields, Gloves
• Piktogramy: GHS07
• Výstražné slovo: Warning
• Kódy nebezpečí: H302,H315,H317
• Bezpečnostní pokyny: P261 - P264 - P272 - P280 - P301 + P312 - P302 + P352
• Hazard Classifications: Acute Tox. 4 Oral - Skin Irrit. 2 - Skin Sens. 1B