79390
Phosphatase, Alkaline from calf intestinal mucosa
suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)
Synonyma:
Alkaline Phosphatase from calf intestinal mucosa
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O této položce
Číslo CAS:
UNSPSC Code:
12352204
eCl@ss:
42010105
EC Number:
232-631-4
NACRES:
NA.54
MDL number:
Specific activity:
~2500 U/mg protein (~10 mg/ml)
Biological source:
bovine (calf) intestinal mucosa
Technický servis
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Dovolte nám, abychom vám pomohlibiological source
bovine (calf) intestinal mucosa
form
solution (clear, colorless)
specific activity
~2500 U/mg protein (~10 mg/ml)
technique(s)
enzyme immunoassay: suitable
impurities
0.1 mM ZnCl2, 1 mM MgCl2, 3 M NaCl, 30 mM triethanolamine
pH
~7.6
storage temp.
2-8°C
Application
Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochem/physiol Actions
Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.
Preparation Note
The solution can be used without dialysis (for enzyme immuno-assay).
Other Notes
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 °C
Sales restrictions may apply
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Skladovací třída
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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