T4661

Trizma^® base

≥99.9% (titration), crystalline

• Synonyma: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Lineární vzorec: NH₂C(CH₂OH)₃
• Číslo CAS: 77-86-1
• Molekulová hmotnost: 121.14
• UNSPSC Code: 12352104
• NACRES: NA.25
• PubChem Substance ID: 24900061
• EC Number: 201-064-4
• Beilstein/REAXYS Number: 741883
• MDL number: MFCD00004679

Vlastnosti

• description: aminopeptidase substrate
• Quality Segment: 400
• assay: ≥99.9% (titration)
• form: crystalline
• loss: ≤5% (loss on drying, 110 °C)
• color: white
• pH: 10.0-11.5
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: soluble (400 g plus 600 mL of water)
• λ: (290nm < 0.05), in 5 mM Tris; DNA (40% (w/w))
• application(s): diagnostic assay manufacturing
• storage temp.: room temp
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Popis

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application

Tris base has been used:

• As a component of lysis buffer for cell disruption
• In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols

Features and Benefits

Features:

1. Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
2. Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
3. M-Clarity^™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity^™ Program
4. Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
5. Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
6. Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
   1. Site quality assessment
   2. ISO 9001 certificate
   3. Detailed product specifications
   4. Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)

Benefits:

1. Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
2. Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
3. Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
4. Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
5. Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
6. Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.

Packaging

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Analysis Note

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Skladovací třída: 11 - Combustible Solids
• Co se děje?: WGK 1
• Bod vzplanutí (°F): Not applicable
• Bod vzplanutí (°C): Not applicable
• PPE (osobní ochranné prostředky): dust mask type N95 (US), Eyeshields, Gloves